UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling

Dobreva, MP; Escalona, VA; Lawson, KA; Sanchez, MN; Ponomarev, LC; Pereira, PNG; Stryjewska, A; ... Zwijsen, A; + view all (2018) Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling. Development , 145 (13) , Article dev157222. 10.1242/dev.157222. Green open access

[img]
Preview
Text
Joyce_dev157222.full.pdf - Published version

Download (7MB) | Preview

Abstract

Upon gastrulation, the mammalian conceptus transforms rapidly from a simple bilayer into a multilayered embryo enveloped by its extra-embryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single-cell labelling and clonal analysis. We identified four clone types with distinct clonal growth patterns in amniotic ectoderm. Two main types have progenitors in extreme proximal-anterior epiblast. Early descendants initiate and expand amniotic ectoderm posteriorly, while descendants of cells remaining anteriorly later expand amniotic ectoderm from its anterior side. Amniogenesis is abnormal in embryos deficient in the bone morphogenetic protein (BMP) signalling effector SMAD5, with delayed closure of the proamniotic canal, and aberrant amnion and folding morphogenesis. Transcriptomics of individual Smad5 mutant amnions isolated before visible malformations and tetraploid chimera analysis revealed two amnion defect sets. We attribute them to impairment of progenitors of the two main cell populations in amniotic ectoderm and to compromised cuboidal-to-squamous transition of anterior amniotic ectoderm. In both cases, SMAD5 is crucial for expanding amniotic ectoderm rapidly into a stretchable squamous sheet to accommodate exocoelom expansion, axial growth and folding morphogenesis.

Type: Article
Title: Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.157222
Publisher version: https://doi.org/10.1242/dev.157222
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Amnion, Amnion fate map, BMP-SMAD, Chorion, Clonal analysis, Extra-embryonic ectoderm, Extra-embryonic-embryonic interface, PRIMORDIAL GERM-CELLS, ANTERIOR VISCERAL ENDODERM, EXTRAEMBRYONIC ECTODERM, GENOMIC FEATURES, BMP2 EXPRESSION, CLONAL ANALYSIS, RNA-SEQ, EMBRYO, DEFECTS, MORPHOGENESIS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research
URI: https://discovery.ucl.ac.uk/id/eprint/10056374
Downloads since deposit
12Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item