Dobreva, MP;
Escalona, VA;
Lawson, KA;
Sanchez, MN;
Ponomarev, LC;
Pereira, PNG;
Stryjewska, A;
... Zwijsen, A; + view all
(2018)
Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling.
Development
, 145
(13)
, Article dev157222. 10.1242/dev.157222.
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Abstract
Upon gastrulation, the mammalian conceptus transforms rapidly from a simple bilayer into a multilayered embryo enveloped by its extra-embryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single-cell labelling and clonal analysis. We identified four clone types with distinct clonal growth patterns in amniotic ectoderm. Two main types have progenitors in extreme proximal-anterior epiblast. Early descendants initiate and expand amniotic ectoderm posteriorly, while descendants of cells remaining anteriorly later expand amniotic ectoderm from its anterior side. Amniogenesis is abnormal in embryos deficient in the bone morphogenetic protein (BMP) signalling effector SMAD5, with delayed closure of the proamniotic canal, and aberrant amnion and folding morphogenesis. Transcriptomics of individual Smad5 mutant amnions isolated before visible malformations and tetraploid chimera analysis revealed two amnion defect sets. We attribute them to impairment of progenitors of the two main cell populations in amniotic ectoderm and to compromised cuboidal-to-squamous transition of anterior amniotic ectoderm. In both cases, SMAD5 is crucial for expanding amniotic ectoderm rapidly into a stretchable squamous sheet to accommodate exocoelom expansion, axial growth and folding morphogenesis.
| Type: | Article |
|---|---|
| Title: | Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1242/dev.157222 |
| Publisher version: | https://doi.org/10.1242/dev.157222 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Amnion, Amnion fate map, BMP-SMAD, Chorion, Clonal analysis, Extra-embryonic ectoderm, Extra-embryonic-embryonic interface, PRIMORDIAL GERM-CELLS, ANTERIOR VISCERAL ENDODERM, EXTRAEMBRYONIC ECTODERM, GENOMIC FEATURES, BMP2 EXPRESSION, CLONAL ANALYSIS, RNA-SEQ, EMBRYO, DEFECTS, MORPHOGENESIS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10056374 |
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