Parekh, M;
Ahmad, S;
Ruzza, A;
Ferrari, S;
(2017)
Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment.
Scientific Reports
, 7
, Article 142. 10.1038/s41598-017-00209-5.
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Abstract
Human corneal endothelial cells (HCEnCs) are responsible for maintaining the transparency of the cornea. Damaged or diseased HCEnCs may cause blindness. Replacement of the diseased cells with a healthy donor endothelium is the only currently available treatment. Tissue-engineering can serve as an alternative to conventional donor corneal transplantation. Due to the global shortage of donor corneas, a wide interest in the development of cultured graft substitutes and artificial corneas has increased. Availability of the old donor corneas is higher especially for research. Although it can be proposed as a valuable source for cell culture, its less proliferative capability emerges a challenge for the researchers. This article describes the use of hyaluronic acid (HA) in combination with Rho-kinase inhibitor (ROCK) Y-27632 for the cultivation of HCEnCs from older donor corneas (age > 60 years). Four conditions including and excluding HA + ROCK and its effect on early attachment rates and proliferation was studied on forty-eight corneas. It was observed that HCEnCs reach confluence within 10–15 days when cultured with HA + ROCK. This approach improves the efficiency of cell adhesion due to force attachment. HCEnCs from old donor corneas can be cultured using this method which may further lead to cell-based therapy for treating corneal endothelial dysfunction.
| Type: | Article |
|---|---|
| Title: | Human Corneal Endothelial Cell Cultivation From Old Donor Corneas With Forced Attachment |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41598-017-00209-5 |
| Publisher version: | https://doi.org/10.1038/s41598-017-00209-5 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, ASCORBIC-ACID 2-PHOSPHATE, PENETRATING KERATOPLASTY, HYALURONAN RECEPTOR, ALLOGRAFT-REJECTION, ROCK INHIBITOR, PROTEIN-KINASE, TRANSPLANTATION, MIGRATION, AGE, OPHTHALMOLOGY |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10055320 |
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