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Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation

Morgan, AD; Ng, YL; Odlyha, M; Gulabivala, K; Bozec, L; (2019) Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation. International Endodontic Journal , 52 (3) pp. 359-370. 10.1111/iej.13004. Green open access

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Abstract

AIM: To establish a method using Fourier Transform Infra‐Red spectroscopy (FTIR) to characterize the nature and depth of changes in dentinal collagen following exposure to sodium hypochlorite (NaOCl) during root canal irrigation in an ex vivo model. METHODOLOGY: Fourier Transform Infra‐Red spectroscopy was used to assess the changes in dentinal collagen when the root canal was exposed to NaOCl. The changes in dentinal collagen caused by NaOCl irrigation of root canals in transverse sections of roots, at 0.5 mm from the canal wall and 0.5 mm from the external root surface, were assessed by FTIR. The data were analysed using paired t‐test with 5% significance level. RESULTS: Fourier Transform Infra‐Red spectroscopy confirmed that NaOCl exposure caused alterations in the chemistry and structure of collagen in dentine. FTIR spectra obtained from dentine surfaces and dentine adjacent to root canals exposed to NaOCl, all consistently showed degradation and conformational change of the collagen structure. FTIR data from the ex vivo model showed that the depth of effect of NaOCl extended to at least 0.5 mm from the canal wall. CONCLUSION: In extracted human teeth, NaOCl caused changes in dentinal collagen that were measurable by FTIR. In an ex vivo model, the depth of effect into dentine extended at least 0.5 mm from the canal wall.

Type: Article
Title: Proof‐of‐concept study to establish an in situ method to determine the nature and depth of collagen changes in dentine using Fourier Transform Infra‐Red spectroscopy after sodium hypochlorite irrigation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/iej.13004
Publisher version: https://doi.org/10.1111/iej.13004
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: collagen, dentine, Fourier Transform Infra‐Red spectroscopy, sodium hypochlorite
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Restorative Dental Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10054760
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