Choe, KY;
Sanchez, CF;
Harris, NG;
Otis, TS;
Mathews, PJ;
(2018)
Optogenetic fMRI and electrophysiological identification of region-specific connectivity between the cerebellar cortex and forebrain.
NeuroImage
, 173
pp. 370-383.
10.1016/j.neuroimage.2018.02.047.
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Abstract
Complex animal behavior is produced by dynamic interactions between discrete regions of the brain. As such, defining functional connections between brain regions is critical in gaining a full understanding of how the brain generates behavior. Evidence suggests that discrete regions of the cerebellar cortex functionally project to the forebrain, mediating long-range communication potentially important in motor and non-motor behaviors. However, the connectivity map remains largely incomplete owing to the challenge of driving both reliable and selective output from the cerebellar cortex, as well as the need for methods to detect region specific activation across the entire forebrain. Here we utilize a paired optogenetic and fMRI (ofMRI) approach to elucidate the downstream forebrain regions modulated by activating a region of the cerebellum that induces stereotypical, ipsilateral forelimb movements. We demonstrate with ofMRI, that activating this forelimb motor region of the cerebellar cortex results in functional activation of a variety of forebrain and midbrain areas of the brain, including the hippocampus and primary motor, retrosplenial and anterior cingulate cortices. We further validate these findings using optogenetic stimulation paired with multi-electrode array recordings and post-hoc staining for molecular markers of activated neurons (i.e. c-Fos). Together, these findings demonstrate that a single discrete region of the cerebellar cortex is capable of influencing motor output and the activity of a number of downstream forebrain as well as midbrain regions thought to be involved in different aspects of behavior.
| Type: | Article |
|---|---|
| Title: | Optogenetic fMRI and electrophysiological identification of region-specific connectivity between the cerebellar cortex and forebrain |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.neuroimage.2018.02.047 |
| Publisher version: | http://doi.org/10.1016/j.neuroimage.2018.02.047 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Cerebellum, ofMRI, Motor cortex, Prefrontal cortex, Thalamus, Hippocampus |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > The Sainsbury Wellcome Centre |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10053610 |
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