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The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: an in vivo murine and in vitro study

Skalicka-Woźniak, K; Walasek, M; Aljarba, T; Stapleton, P; Gibbons, S; Xiao, J; Łuszczki, J; (2018) The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: an in vivo murine and in vitro study. Food and Chemical Toxicology , 120 pp. 472-478. 10.1016/j.fct.2018.07.045. Green open access

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Abstract

The high-performance counter-current chromatography was used for the efficient purification of single constituents from Thymus vulgaris essential oil. Mixtures of n-heptane, ethyl acetate, methanol, and water (5:2:5:2 and 4:1:4:1 v/v), allowed purification of eugenol, 1-octen-3- ol, borneol, thymol, terpinen-4-ol, and camphor, while n-hexane, acetonitrile, and tert-butyl methyl ether (1:1:0.1 v/v) yielded carvacrol, borneol, linalyl acetate, caryophyllene oxide, p-cymene, and eucalyptol. The anticonvulsant activities were evaluated in the maximal electroshock-induced seizure test in mice model (systemic i. p. administration). The oil exerted protection against MES-induced seizures when administered 15 and 30 min before the tests (50 and 62.5%, respectively). Among the isolates, borneol, thymol, and eugenol exerted the strongest protection against seizures. Moreover, linalool had the ability to reduce the transfer of the pKM101 plasmid by 84%, what has the potential to reduce virulence and resistance spread in E. coli. No acute toxic effects towards the CNS were noticed either for the essential oil or for single compounds, in the chimney and grip-strength tests. The preclinical screening of Thymus vulgaris EO, as well as isolated terpenoids, provides evidence that the EO has partial protective activity against seizures and HPCCC technique is suitable for its large scale isolation.

Type: Article
Title: The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: an in vivo murine and in vitro study
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.fct.2018.07.045
Publisher version: https://doi.org/10.1016/j.fct.2018.07.045
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Epilepsy, Seizure, Natural products, Terpenes, Isolation, Purification, Anti-conjugation, Escherichia coli
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10053236
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