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Direct and efficient transfection of mouse neural stem cells and mature neurons by in vivo mRNA electroporation

Bugeon, S; de Chevigny, A; Boutin, C; Core, N; Wild, S; Bosio, A; Cremer, H; (2017) Direct and efficient transfection of mouse neural stem cells and mature neurons by in vivo mRNA electroporation. Development , 144 (21) pp. 3968-3977. 10.1242/dev.151381. Green open access

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Abstract

In vivo brain electroporation of DNA expression vectors is a widely used method for lineage and gene function studies in the developing and postnatal brain. However, transfection efficiency of DNA is limited and adult brain tissue is refractory to electroporation. Here, we present a systematic study of mRNA as a vector for acute genetic manipulation in the developing and adult brain. We demonstrate that mRNA electroporation is far more efficient than DNA electroporation, and leads to faster and more homogeneous protein expression in vivo. Importantly, mRNA electroporation allows the manipulation of neural stem cells and postmitotic neurons in the adult brain using minimally invasive procedures. Finally, we show that this approach can be efficiently used for functional studies, as exemplified by transient overexpression of the neurogenic factor Myt1l and by stably inactivating Dicer nuclease in vivo in adult born olfactory bulb interneurons and in fully integrated cortical projection neurons.

Type: Article
Title: Direct and efficient transfection of mouse neural stem cells and mature neurons by in vivo mRNA electroporation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.151381
Publisher version: https://doi.org/10.1242/dev.151381
Language: English
Additional information: This is the published version of record. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10053131
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