Huang, D; Freeley, M; Matteo, P; (2015) Controlled Organization of DNA Origami on Nano-Patterned Surfaces. Presented at: 12th International Conference on Materials Chemistry: MC 12, University of York, UK.

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Abstract

DNA assembles according to precise base-pairing rules. This allows for the construction of DNA nanostructures (origami) in which single- or double-stranded DNA is folded into various shapes with a high degree of the positional order allowing for complex arrangements to be obtained. In addition, DNA origami can be employed as scaffolds to pattern bioactive structures and nanostructures (e.g. Quantum Dots, CNTs etc.) at the scale of individual molecules. In order to take full advantage of this approach, we need to control the organization of DNA origami on surfaces. This can allow for the fabrication of biochips for single-molecule investigations. Here we present novel strategies to organize DNA nanostructures on nano-patterned surfaces with nanoscale resolution. Focus Ion Beam (FIB) was employed to pattern nano-arrays on silicon dioxide substrates. The exposed areas were chemically modified via the controlled formation of self-assembled monolayers (SAMs) exhibiting DNA origami-binding head groups. DNA nanostructures were then tethered to the nano-patterned areas via covalent bonding, allowing us to control their orientation on substrates. Investigations were carried out via fluorescence microscopy, scanning electron microscopy (SEM) and scanning probe microscopy approaches (atomic force microscopy, AFM) in order to confirm the controlled organization of DNA origami on surfaces. This approach is of general applicability for the fabrication of biochips for bimolecular investigations.

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