de Wit, G;
Albrecht, D;
Ewers, H;
Kukura, P;
(2018)
Revealing Compartmentalized Diffusion in Living Cells with Interferometric Scattering Microscopy.
Biophysical Journal
, 114
(12)
pp. 2945-2950.
10.1016/j.bpj.2018.05.007.
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Abstract
The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background suppression, at the expense of imaging speed and observation time. Here, we show that interferometric scattering microscopy combined with 40 nm gold nanoparticle labeling can be used to follow the motion of membrane proteins in the plasma membrane of live cultured mammalian cell lines and hippocampal neurons with up to 3 nm precision and 25 μs temporal resolution. The achievable spatiotemporal precision enabled us to reveal signatures of compartmentalization in neurons likely caused by the actin cytoskeleton.
| Type: | Article |
|---|---|
| Title: | Revealing Compartmentalized Diffusion in Living Cells with Interferometric Scattering Microscopy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bpj.2018.05.007 |
| Publisher version: | http://doi.org/10.1016/j.bpj.2018.05.007 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10052649 |
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