Picazo-Bueno, JA;
Cojoc, D;
Iseppon, F;
Torre, V;
Mico, V;
(2018)
Single-shot, dual-mode, water-immersion microscopy platform for biological applications.
Applied Optics
, 57
(1)
A242-A249.
10.1364/AO.57.00A242.
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Abstract
A single-shot water-immersion digital holographic microscope combined with broadband (white light) illumination mode is presented. This double imaging platform allows conventional incoherent visualization with phase holographic imaging of inspected samples. The holographic architecture is implemented at the image space (that is, after passing the microscope lens), thus reducing the sensitivity of the system to vibrations and/or thermal changes in comparison to regular interferometers. Because of the off-axis holographic recording principle, quantitative phase images of live biosamples can be recorded in a single camera snapshot at full-field geometry without any moving parts. And, the use of water-immersion imaging lenses maximizes the achievable resolution limit. This dual-mode microscope platform is first calibrated using microbeads, then applied to the characterization of fixed cells (neuroblastoma, breast cancer, and hippocampal neuronal cells) and, finally, validated for visualization of dynamic living cells (hippocampal neurons).
| Type: | Article |
|---|---|
| Title: | Single-shot, dual-mode, water-immersion microscopy platform for biological applications |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1364/AO.57.00A242 |
| Publisher version: | http://dx.doi.org/10.1364/AO.57.00A242 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Physical Sciences, Optics, DIGITAL HOLOGRAPHIC MICROSCOPY, PHASE-CONTRAST MICROSCOPY, OFF-AXIS HOLOGRAMS, REFRACTIVE-INDEX, LIVING CELLS, COMMON-PATH, SUPERRESOLUTION, INTERFEROMETRY, SAMPLES, COHERENCE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10052273 |
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