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Single-shot, dual-mode, water-immersion microscopy platform for biological applications

Picazo-Bueno, JA; Cojoc, D; Iseppon, F; Torre, V; Mico, V; (2018) Single-shot, dual-mode, water-immersion microscopy platform for biological applications. Applied Optics , 57 (1) A242-A249. 10.1364/AO.57.00A242. Green open access

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Abstract

A single-shot water-immersion digital holographic microscope combined with broadband (white light) illumination mode is presented. This double imaging platform allows conventional incoherent visualization with phase holographic imaging of inspected samples. The holographic architecture is implemented at the image space (that is, after passing the microscope lens), thus reducing the sensitivity of the system to vibrations and/or thermal changes in comparison to regular interferometers. Because of the off-axis holographic recording principle, quantitative phase images of live biosamples can be recorded in a single camera snapshot at full-field geometry without any moving parts. And, the use of water-immersion imaging lenses maximizes the achievable resolution limit. This dual-mode microscope platform is first calibrated using microbeads, then applied to the characterization of fixed cells (neuroblastoma, breast cancer, and hippocampal neuronal cells) and, finally, validated for visualization of dynamic living cells (hippocampal neurons).

Type: Article
Title: Single-shot, dual-mode, water-immersion microscopy platform for biological applications
Open access status: An open access version is available from UCL Discovery
DOI: 10.1364/AO.57.00A242
Publisher version: http://dx.doi.org/10.1364/AO.57.00A242
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Physical Sciences, Optics, DIGITAL HOLOGRAPHIC MICROSCOPY, PHASE-CONTRAST MICROSCOPY, OFF-AXIS HOLOGRAMS, REFRACTIVE-INDEX, LIVING CELLS, COMMON-PATH, SUPERRESOLUTION, INTERFEROMETRY, SAMPLES, COHERENCE
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research
URI: https://discovery.ucl.ac.uk/id/eprint/10052273
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