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Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism

Khodadoost, L; Hussain, H; Mullany, P; (2017) Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism. FEMS Microbiology Letters , 364 (21) , Article fnx208. 10.1093/femsle/fnx208. Green open access

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Mullany_Plasmids can transfer to Clostridium difficile CD37 and 630 Delta erm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism_AAM.pdf - Accepted version

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Mullany_Plasmids can transfer to Clostridium difficile CD37 and 630 Delta erm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism_Table_1.pdf - Accepted version

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Mullany_Plasmids can transfer to Clostridium difficile CD37 and 630 Delta erm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism_Table_2.pdf - Accepted version

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Abstract

Broad host range conjugative plasmids that replicate in Escherichia coli have been widely used to mobilise smaller replicons, bearing their cognate origin of transfer (oriT) into a variety of organisms that are less tractable genetically, such as Clostridium (Clostridioides) difficile. In this work we demonstrated that the oriT region of pMTL9301 (derived from RK2) is not required for transfer between E. coli and C. difficile strains 630Δerm and CD37 and that this oriT-independent transfer is abolished in the presence of DNase when CD37 is the recipient. Transfer to the 630Δerm strain is DNase resistant even without an obvious oriT, when E. coli CA434 is used as a donor and is sensitive to DNase when E. coli HB101 is the donor.

Type: Article
Title: Plasmids can transfer to Clostridium difficile CD37 and 630Δerm both by a DNase resistant conjugation-like mechanism and a DNase sensitive mechanism
Open access status: An open access version is available from UCL Discovery
DOI: 10.1093/femsle/fnx208
Publisher version: https://doi.org/10.1093/femsle/fnx208
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Clostridium difficile, horizontal gene transfer, cell–cell transformation, plasmid, conjugative transposon
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10052150
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