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Knocking down Stard3 decreases adipogenesis with decreased mitochondrial ROS in 3T3-L1 cells

Zhou, X; Gao, H; Guo, Y; Chen, Y; Ruan, XZ; (2018) Knocking down Stard3 decreases adipogenesis with decreased mitochondrial ROS in 3T3-L1 cells. Biochemical and Biophysical Research Communications , 504 (2) pp. 387-392. 10.1016/j.bbrc.2018.06.030. Green open access

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Abstract

Start domain-containing protein 3 (Stard3) plays roles in intracellular cholesterol distribution, however, the role of Stard3 in the adipogenesis of 3T3-L1 preadipocytes remains unclear. We demonstrated that Stard3 expression was significantly increased during the adipogenesis of 3T3-L1 preadipocytes, accompanied by an increase of mitochondrial Reactive oxygen species (ROS). Stard3 knocking-down inhibited 3T3-L1 preadipocyte adipogenesis with decreased mitochondrial ROS levels, while ROS inducer rescued the stard3 silencing 3T3 cells with increased ROS. Moreover, Stard3 silencing reduced the expression of peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein (C/EBP)α in 3T3- L1 cells. In conclusion, Stard3 enhanced the adipogenesis of preadipocytes by enhancement of cholesterol redistribution to the mitochondrial, increasing mitochondrial ROS production. These results suggest that Stard3 is an essential factor for the 3T3-L1 cells' differentiation.

Type: Article
Title: Knocking down Stard3 decreases adipogenesis with decreased mitochondrial ROS in 3T3-L1 cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bbrc.2018.06.030
Publisher version: http://dx.doi.org/10.1016/j.bbrc.2018.06.030
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Adipogenesis, Cholesterol, Start domain-containing protein 3
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Renal Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10051410
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