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SRRF: Universal live-cell super-resolution microscopy

Culley, S; Tosheva, KL; Matos Pereira, P; Henriques, R; (2018) SRRF: Universal live-cell super-resolution microscopy. International Journal of Biochemistry & Cell Biology , 101 pp. 74-79. 10.1016/j.biocel.2018.05.014. Green open access

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Abstract

Super-resolution microscopy techniques break the diffraction limit of conventional optical microscopy to achieve resolutions approaching tens of nanometres. The major advantage of such techniques is that they provide resolutions close to those obtainable with electron microscopy while maintaining the benefits of light microscopy such as a wide palette of high specificity molecular labels, straightforward sample preparation and live-cell compatibility. Despite this, the application of super-resolution microscopy to dynamic, living samples has thus far been limited and often requires specialised, complex hardware. Here we demonstrate how a novel analytical approach, Super-Resolution Radial Fluctuations (SRRF), is able to make live-cell super-resolution microscopy accessible to a wider range of researchers. We show its applicability to live samples expressing GFP using commercial confocal as well as laser- and LED-based widefield microscopes, with the latter achieving long-term timelapse imaging with minimal photobleaching.

Type: Article
Title: SRRF: Universal live-cell super-resolution microscopy
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.biocel.2018.05.014
Publisher version: https://doi.org/10.1016/j.biocel.2018.05.014
Language: English
Additional information: Copyright © 2018 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Keywords: Fluorescence, Image processing, Live-cell imaging, Super-resolution microscopy
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: https://discovery.ucl.ac.uk/id/eprint/10050860
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