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Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Lima, AF; May, G; Colunga, J; Pedreiro, S; Paiva, A; Ferreira, L; Enver, T; ... Pires das Neves, R; + view all (2018) Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation. Scientific Reports , 8 , Article 7210. 10.1038/s41598-018-25517-2. Green open access

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Abstract

Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34+ umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.

Type: Article
Title: Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41598-018-25517-2
Publisher version: http://dx.doi.org/10.1038/s41598-018-25517-2
Language: English
Additional information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, Pluripotent Stem-Cells, Umbilical-Cord Blood, Small-Molecule Compounds, RNA-Polymerase-II, Mammalian-Cells, Transcription Factors, Hypotonic Stress, Cd34(+) Cells, Binding, DNA
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/10050804
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