Andersson, SEM;
Eneljung, T;
Tengvall, S;
Jirholt, P;
Stern, A;
Henningsson, L;
Liang, B;
... Gjertsson, I; + view all
(2016)
Collagen epitope expression on B cells is sufficient to confer tolerance to collagen-induced arthritis.
Arthritis Research & Therapy
, 18
, Article 140. 10.1186/s13075-016-1037-7.
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Abstract
Background: The mechanisms underlying tolerance induction and maintenance in autoimmune arthritis remain elusive. In a mouse model of rheumatoid arthritis, collagen type II (CII)-induced arthritis, we explore the contribution of B cells to antigen-specific tolerance. Methods: To generate expression of the CII-peptide specifically on B-cell major histocompatibility complex type II, lentiviral-based gene therapy including a B-cell-specific Igk promoter was used. Results: Presentation of the CII-peptide on B cells significantly reduced the frequency and severity of arthritis as well as the serum levels of CII -specific IgG antibodies. Further, both frequency and suppressive function of regulatory T cells were increased in tolerized mice. Adoptive transfer of regulatory T cells from tolerized mice to naïve mice ameliorated the development of CII-induced arthritis. Conclusion: Our data suggest that endogenous presentation of the CII-peptide on B cells is one of the key contributors to arthritis tolerance induction and maintenance.
Type: | Article |
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Title: | Collagen epitope expression on B cells is sufficient to confer tolerance to collagen-induced arthritis |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1186/s13075-016-1037-7 |
Publisher version: | http://doi.org/10.1186/s13075-016-1037-7 |
Language: | English |
Additional information: | © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
Keywords: | Tolerance, B cells, B lymphocytes, Arthritis, Collagen, Collagen type II, Gene therapy, Antigen presentation |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
URI: | https://discovery.ucl.ac.uk/id/eprint/10050773 |
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