Bawn, M;
Subrizi, F;
Lye, GJ;
Sheppard, TD;
Hailes, HC;
Ward, JM;
(2018)
One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose.
Enzyme and Microbial Technology
, 116
pp. 16-22.
10.1016/j.enzmictec.2018.05.006.
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Abstract
The use of biocatalysis for the synthesis of high value added chemical building blocks derived from biomass is becoming an increasingly important application for future sustainable technologies. The synthesis of a higher value chemical from L-arabinose, the predominant monosaccharide obtained from sugar beet pulp, is demonstrated here via a transketolase and transaminase coupled reaction. Thermostable transketolases derived from Deinococcus geothermalis and Dei nococcus radiodurans catalysed the synthesis of L-gluco-heptulose from L-arabinose and β-hydroxypyruvate at elevated temperatures with high conversions. β-Hydroxypyruvate, a commercially expensive compound used in the transketolase reaction, was generated in situ from L-serine and α-ketoglutaric acid via a thermostable transaminase, also from Deinococcus geothermalis. The two steps were investigated and implemented in a one-pot system for the sustainable and efficient production of L-gluco-heptulose.
| Type: | Article |
|---|---|
| Title: | One-pot, two-step transaminase and transketolase synthesis of L-gluco-heptulose from L-arabinose |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.enzmictec.2018.05.006 |
| Publisher version: | https://doi.org/10.1016/j.enzmictec.2018.05.006 |
| Language: | English |
| Additional information: | © 2018 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | l-Arabinose, Transaminase, Transketolase, Hydroxypyruvate, Thermostable, l-Gluco-heptulose |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10050122 |
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