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XNA ligation using T4 DNA ligase in crowding conditions

Kestemont, D; Renders, M; Leonczak, P; Abramov, M; Schepers, G; Pinheiro, VB; Rozenski, J; (2018) XNA ligation using T4 DNA ligase in crowding conditions. Chemical Communications , 54 (49) pp. 6408-6411. 10.1039/C8CC02414F. Green open access

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Abstract

T4 DNA ligase is capable of ligating 2′OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

Type: Article
Title: XNA ligation using T4 DNA ligase in crowding conditions
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/C8CC02414F
Publisher version: https://doi.org/10.1039/C8CC02414F
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10049874
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