Parker, GJ;
Leppert, T;
Anex, DS;
Hilmer, JK;
Matsunami, N;
Baird, L;
Stevens, J;
... Leppert, M; + view all
(2016)
Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome.
PLoS One
, 11
(9)
, Article e0160653. 10.1371/journal.pone.0160653.
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Abstract
Human identification from biological material is largely dependent on the ability to characterize genetic polymorphisms in DNA. Unfortunately, DNA can degrade in the environment, sometimes below the level at which it can be amplified by PCR. Protein however is chemically more robust than DNA and can persist for longer periods. Protein also contains genetic variation in the form of single amino acid polymorphisms. These can be used to infer the status of non-synonymous single nucleotide polymorphism alleles. To demonstrate this, we used mass spectrometry-based shotgun proteomics to characterize hair shaft proteins in 66 European-American subjects. A total of 596 single nucleotide polymorphism alleles were correctly imputed in 32 loci from 22 genes of subjects' DNA and directly validated using Sanger sequencing. Estimates of the probability of resulting individual non-synonymous single nucleotide polymorphism allelic profiles in the European population, using the product rule, resulted in a maximum power of discrimination of 1 in 12,500. Imputed non-synonymous single nucleotide polymorphism profiles from European-American subjects were considerably less frequent in the African population (maximum likelihood ratio = 11,000). The converse was true for hair shafts collected from an additional 10 subjects with African ancestry, where some profiles were more frequent in the African population. Genetically variant peptides were also identified in hair shaft datasets from six archaeological skeletal remains (up to 260 years old). This study demonstrates that quantifiable measures of identity discrimination and biogeographic background can be obtained from detecting genetically variant peptides in hair shaft protein, including hair from bioarchaeological contexts.
| Type: | Article |
|---|---|
| Title: | Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0160653 |
| Publisher version: | https://doi.org/10.1371/journal.pone.0160653 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | African Continental Ancestry Group, Alleles, European Continental Ancestry Group, Forensic Anthropology, Genotype, Hair, Humans, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Proteomics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10049282 |
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