Basso-Ricci, L;
Scala, S;
Milani, R;
Migliavacca, M;
Rovelli, A;
Bernardo, ME;
Ciceri, F;
... Biasco, L; + view all
(2017)
Multiparametric Whole Blood Dissection: A one-shot comprehensive picture of the human hematopoietic system.
Cytometry Part A
, 91
(10)
pp. 952-965.
10.1002/cyto.a.23148.
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Abstract
Human hematopoiesis is a complex and dynamic system where morphologically and functionally diverse mature cell types are generated and maintained throughout life by bone marrow (BM) Hematopoietic Stem/Progenitor Cells (HSPC). Congenital and acquired hematopoietic disorders are often diagnosed through the detection of aberrant frequency or composition of hematopoietic cell populations. We here describe a novel protocol, called “Whole Blood Dissection” (WBD), capable of analyzing in a single test‐tube, hematopoietic progenitors and all major mature cell lineages composing either BM or peripheral blood (PB) through a multiparametric flow‐cytometry analysis. WBD allows unambiguously identifying in the same tube up to 23 different blood cell types including HSPC subtypes and all the major myeloid and lymphoid lineage compartments at different stages of maturation, through a combination of 17 surface and 1 viability cell markers. We assessed the efficacy of WBD by analyzing BM and PB samples from adult (n = 8) and pediatric (n = 9) healthy donors highlighting age‐related shift in cell composition. We also tested the capability of WBD on detecting aberrant hematopoietic cell composition in clinical samples of patients with primary immunodeficiency or leukemia unveiling expected and novel hematopoietic unbalances. Overall, WBD allows unambiguously identifying >99% of the cell subpopulations composing a blood sample in a reproducible, standardized, cost‐, and time‐efficient manner. This tool has a wide range of potential pre‐clinical and clinical applications going from the characterization of hematopoietic disorders to the monitoring of hematopoietic reconstitution in patients after transplant or gene therapy.
| Type: | Article |
|---|---|
| Title: | Multiparametric Whole Blood Dissection: A one-shot comprehensive picture of the human hematopoietic system |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/cyto.a.23148 |
| Publisher version: | https://doi.org/10.1002/cyto.a.23148 |
| Language: | English |
| Additional information: | Copyright © 2017 The Authors. This is an open access article under the terms of the Creative Commons AttributionNonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution and reproduction in any medium, provided that the Contribution is properly cited and is not used for commercial purposes. |
| Keywords: | hematopoiesis; hematopoietic stem cells; flow-cytometry; leukemia; immunodeficiency |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10048991 |
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