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A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems

Wiegmann, V; Martinez, CB; Baganz, F; (2018) A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems. Biotechnology Letters , 40 (7) pp. 1029-1036. 10.1007/s10529-018-2556-x. Green open access

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Abstract

Objectives: Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. Results: A correlation between evaporation and the concentration of Na + was found (R 2 = 0.95) when using the 24-well-based miniature bioreactor system (micro-Matrix) for a batch culture with GS-CHO. Based on these results, a method was developed to counteract evaporation with periodic water additions based on measurements of the Na + concentration. Implementation of this method resulted in a reduction of the relative liquid loss after 15 days of a fed-batch cultivation from 36.7 ± 6.7% without volume corrections to 6.9 ± 6.5% with volume corrections. Conclusion: A procedure was established to indirectly measure evaporation through a correlation with the level of Na + ions in solution and deriving a simple formula to account for liquid losses.

Type: Article
Title: A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s10529-018-2556-x
Publisher version: http://doi.org/10.1007/s10529-018-2556-x
Language: English
Additional information: Copyright © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Keywords: Evaporation, Fed batch, GS-CHO cells, Miniature shaken bioreactor, Reproducibility
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10048698
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