Garcia-Caballero, A;
Zhang, FX;
Hodgkinson, V;
Huang, J;
Chen, L;
Souza, IA;
Cain, S;
... Zamponi, GW; + view all
(2018)
T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B.
Molecular Brain
, 11
, Article 24. 10.1186/s13041-018-0368-5.
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Abstract
This study describes the functional interaction between the Cav3.1 and Cav3.2 T-type calcium channels and cytoskeletal spectrin (α/β) and ankyrin B proteins. The interactions were identified utilizing a proteomic approach to identify proteins that interact with a conserved negatively charged cytosolic region present in the carboxy-terminus of T-type calcium channels. Deletion of this stretch of amino acids decreased binding of Cav3.1 and Cav3.2 calcium channels to spectrin (α/β) and ankyrin B and notably also reduced T-type whole cell current densities in expression systems. Furthermore, fluorescence recovery after photobleaching analysis of mutant channels lacking the proximal C-terminus region revealed reduced recovery of both Cav3.1 and Cav3.2 mutant channels in hippocampal neurons. Knockdown of spectrin α and ankyrin B decreased the density of endogenous Cav3.2 in hippocampal neurons. These findings reveal spectrin (α/β) / ankyrin B cytoskeletal and signaling proteins as key regulators of T-type calcium channels expressed in the nervous system.
| Type: | Article |
|---|---|
| Title: | T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/s13041-018-0368-5 |
| Publisher version: | http://doi.org/10.1186/s13041-018-0368-5 |
| Language: | English |
| Additional information: | Copyright © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| Keywords: | T-type channels, Spectrin (α/β), Ankyrin B, Trafficking, Cav3.1, Cav3.2 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10048491 |
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