Ryabova, AV;
Keevend, K;
Tsolaki, E;
Bertazzo, S;
Pominova, DV;
Romanishkin, ID;
Grachev, PV;
... Orlovskii, YV; + view all
(2018)
Visualization of Nd3+-doped Laf3 Nanoparticles
For Near Infrared Bioimaging via Upconversion
Luminescence at Multiphoton Excitation
Microscopyvisualization of Nd3+-doped Laf3 Nanoparticles
For Near Infrared Bioimaging via Upconversion
Luminescence at Multiphoton Excitation
Microscopy.
Biomedical Photonics
, 7
(1)
pp. 4-12.
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Abstract
Recent developments in the field of biophotonics facilitate the raise of interest to inorganic nanoparticles (NPs) doped with Nd 3+ ions, because of their near-infrared (NIR) absorption. These NPs are interesting bioimaging probes for deep tissue visualization, while they can also act as local thermometers in biological tissues. Despite the good possibilities for visualization of NPs with Nd 3+ ions in NIR spectral range, difficulties arise when studying the cellular uptake of these NPs using commercially available fluorescence microscopy systems, since the selection of suitable luminescence detectors is limited. However, Nd 3+ ions are able to convert NIR radiation into visible light, showing upconversion properties. In this paper we found optimal parameters to excite upconversion luminescence of Nd 3+ :LaF 3 NPs in living cells and to compare the distribution of the NPs inside the cell culture of human macrophages THP-1 obtained by two methods. Firstly, by detecting the upconversion luminescence of the NPs in VIS under NIR multiphoton excitation using laser scanning confocal microscopy and secondly, using transmission electron microscopy.
| Type: | Article |
|---|---|
| Title: | Visualization of Nd3+-doped Laf3 Nanoparticles For Near Infrared Bioimaging via Upconversion Luminescence at Multiphoton Excitation Microscopyvisualization of Nd3+-doped Laf3 Nanoparticles For Near Infrared Bioimaging via Upconversion Luminescence at Multiphoton Excitation Microscopy |
| Open access status: | An open access version is available from UCL Discovery |
| Publisher version: | http://www.pdt-journal.com/jour/article/download/2... |
| Language: | English |
| Additional information: | Copyright © Authors. The content is available under the Creative Commons Attribution 4.0 License. |
| Keywords: | Nd3+-doped nanoparticles, near-infrared, upconversion luminescence, multiphoton excitation, laser scanning confocal microscopy. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10048476 |
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