UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Isolation of Schwann Cell Precursors from Rodents.

Mirsky, R; Jessen, KR; (2018) Isolation of Schwann Cell Precursors from Rodents. Methods in Molecular Biology , 1739 pp. 3-15. 10.1007/978-1-4939-7649-2_1. Green open access

[img]
Preview
Text
Mirsky_Isolation_Schwann_Cell.pdf - Accepted version

Download (564kB) | Preview

Abstract

Schwann cell precursors are the first defined stage in the generation of Schwann cells from the neural crest and represent the glial cell of embryonic nerves. Highly pure cultures of these cells can be obtained by enzymatic dissociation of nerves dissected from the limbs of 14- or 12-day-old rat and mouse embryos, respectively. Since Schwann cell precursors, unlike Schwann cells, are acutely dependent on axonal signals for survival, they require addition of trophic factors, typically β-neuregulin-1, for maintenance in cell culture. Under these conditions they convert to Schwann cells on schedule, within about 4 days.

Type: Article
Title: Isolation of Schwann Cell Precursors from Rodents.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-7649-2_1
Publisher version: https://doi.org/10.1007/978-1-4939-7649-2_1
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Nerve development, Neural crest, Neuregulin, Precursors, Schwann cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10048371
Downloads since deposit
62Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item