Mirsky, R;
Jessen, KR;
(2018)
Isolation of Schwann Cell Precursors from Rodents.
Methods in Molecular Biology
, 1739
pp. 3-15.
10.1007/978-1-4939-7649-2_1.
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Abstract
Schwann cell precursors are the first defined stage in the generation of Schwann cells from the neural crest and represent the glial cell of embryonic nerves. Highly pure cultures of these cells can be obtained by enzymatic dissociation of nerves dissected from the limbs of 14- or 12-day-old rat and mouse embryos, respectively. Since Schwann cell precursors, unlike Schwann cells, are acutely dependent on axonal signals for survival, they require addition of trophic factors, typically β-neuregulin-1, for maintenance in cell culture. Under these conditions they convert to Schwann cells on schedule, within about 4 days.
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