Schiffer, PH;
Polsky, AL;
Cole, AG;
Camps, JIR;
Kroiher, M;
Silver, DH;
Grishkevich, V;
... Yanai, I; + view all
(2018)
The gene regulatory program of Acrobeloides nanus reveals conservation of phylum-specific expression.
Proceedings of the National Academy of Sciences of the United States of America
, 115
(17)
pp. 4459-4464.
10.1073/pnas.1720817115.
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Abstract
The evolution of development has been studied through the lens of gene regulation by examining either closely related species or extremely distant animals of different phyla. In nematodes, detailed cell- and stage-specific expression analyses are focused on the model Caenorhabditis elegans, in part leading to the view that the developmental expression of gene cascades in this species is archetypic for the phylum. Here, we compared two species of an intermediate evolutionary distance: the nematodes C. elegans (clade V) and Acrobeloides nanus (clade IV). To examine A. nanus molecularly, we sequenced its genome and identified the expression profiles of all genes throughout embryogenesis. In comparison with C. elegans, A. nanus exhibits a much slower embryonic development and has a capacity for regulative compensation of missing early cells. We detected conserved stages between these species at the transcriptome level, as well as a prominent middevelopmental transition, at which point the two species converge in terms of their gene expression. Interestingly, we found that genes originating at the dawn of the Ecdysozoa supergroup show the least expression divergence between these two species. This led us to detect a correlation between the time of expression of a gene and its phylogenetic age: evolutionarily ancient and young genes are enriched for expression in early and late embryogenesis, respectively, whereas Ecdysozoa-specific genes are enriched for expression during the middevelopmental transition. Our results characterize the developmental constraints operating on each individual embryo in terms of developmental stages and genetic evolutionary history.
| Type: | Article |
|---|---|
| Title: | The gene regulatory program of Acrobeloides nanus reveals conservation of phylum-specific expression |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1073/pnas.1720817115 |
| Publisher version: | http://dx.doi.org/10.1073/pnas.1720817115 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | evolution, development, gene expression, Nematoda, developmental constraints, TRANSPOSABLE ELEMENTS, LIFE-HISTORY, BODY PLANS, RNA-SEQ, EVOLUTION, NEMATODES, EMBRYOS, EMBRYOGENESIS, DIVERGENCE, PATTERNS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Education UCL > Provost and Vice Provost Offices > School of Education > UCL Institute of Education UCL > Provost and Vice Provost Offices > School of Education > UCL Institute of Education > IOE - Psychology and Human Development |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10048202 |
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