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Fundamental aspects of arm repair phase in two echinoderm models

Ferrario, C; Ben Khadra, Y; Czarkwiani, A; Zakrzewski, A; Martinez, P; Colombo, G; Bonasoro, F; ... Sugni, M; + view all (2017) Fundamental aspects of arm repair phase in two echinoderm models. Developmental Biology , 433 (2) pp. 297-309. 10.1016/j.ydbio.2017.09.035. Green open access

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Abstract

Regeneration is a post-embryonic developmental process that ensures complete morphological and functional restoration of lost body parts. The repair phase is a key step for the effectiveness of the subsequent regenerative process: in vertebrates, efficient re-epithelialisation, rapid inflammatory/immune response and post-injury tissue remodelling are fundamental aspects for the success of this phase, their impairment leading to an inhibition or total prevention of regeneration. Among deuterostomes, echinoderms display a unique combination of striking regenerative abilities and diversity of useful experimental models, although still largely unexplored. Therefore, the brittle star Amphiura filiformis and the starfish Echinaster sepositus were here used to comparatively investigate the main repair phase events after injury as well as the presence and expression of immune system and extracellular matrix (i.e. collagen) molecules using both microscopy and molecular tools. Our results showed that emergency reaction and re-epithelialisation are similar in both echinoderm models, being faster and more effective than in mammals. Moreover, in comparison to the latter, both echinoderms showed delayed and less abundant collagen deposition at the wound site (absence of fibrosis). The gene expression patterns of molecules related to the immune response, such as Ese-fib-like (starfishes) and Afi-ficolin (brittle stars), were described for the first time during echinoderm regeneration providing promising starting points to investigate the immune system role in these regeneration models. Overall, the similarities in repair events and timing within the echinoderms and the differences with what has been reported in mammals suggest that effective repair processes in echinoderms play an important role for their subsequent ability to regenerate. Targeted molecular and functional analyses will shed light on the evolution of these abilities in the deuterostomian lineage.

Type: Article
Title: Fundamental aspects of arm repair phase in two echinoderm models
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ydbio.2017.09.035
Publisher version: https://doi.org/10.1016/j.ydbio.2017.09.035
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Starfishes, Brittle stars, Emergency reaction, Wound healing, Collagen, Immune/inflammatory response, CUCUMBER HOLOTHURIA-GLABERRIMA, SEA-URCHIN, PROLYL 4-HYDROXYLASE, ANTEDON-MEDITERRANEA, AMPHIURA-FILIFORMIS, ASTERIAS-RUBENS, INNATE IMMUNITY, WOUND REPAIR, REGENERATION, MECHANISMS
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10047760
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