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Concepts in Light Microscopy of Viruses

Witte, R; Andriasyan, V; Georgi, F; Yakimovich, A; Greber, UF; (2018) Concepts in Light Microscopy of Viruses. Viruses , 10 (4) , Article 202. 10.3390/v10040202. Green open access

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Abstract

Viruses threaten humans, livestock, and plants, and are difficult to combat. Imaging of viruses by light microscopy is key to uncover the nature of known and emerging viruses in the quest for finding new ways to treat viral disease and deepening the understanding of virus–host interactions. Here, we provide an overview of recent technology for imaging cells and viruses by light microscopy, in particular fluorescence microscopy in static and live-cell modes. The review lays out guidelines for how novel fluorescent chemical probes and proteins can be used in light microscopy to illuminate cells, and how they can be used to study virus infections. We discuss advantages and opportunities of confocal and multi-photon microscopy, selective plane illumination microscopy, and super-resolution microscopy. We emphasize the prevalent concepts in image processing and data analyses, and provide an outlook into label-free digital holographic microscopy for virus research.

Type: Article
Title: Concepts in Light Microscopy of Viruses
Location: Switzerland
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/v10040202
Publisher version: https://doi.org/10.3390/v10040202
Language: English
Additional information: Copyright © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: DNA virus, RNA virus, adeno-associated virus AAV, adenovirus, baculovirus, cell biology, computing, cytoskeleton, data analysis, endocytosis, enveloped virus, fluorescence microscopy, fluorescent virions, gene expression, gene therapy, hepatitis B virus, herpes simplex virus, herpesvirus, high-throughput screening, modeling, human immunodeficiency virus HIV, image analysis, immunofluorescence microscopy, infection, influenza virus, innate immunity, internalization, intracellular transport, light microscopy, live imaging, machine learning, membrane traffic, microscopy, nonenveloped virus, parvovirus, quantitative microscopy, receptor, simian virus 40, simulation, super-resolution, trafficking, virion uncoating, virus entry, virus infection, virus labeling
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10047520
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