Bohmer, N;
Demarmels, N;
Tsolaki, E;
Gerken, L;
Keevend, K;
Bertazzo, S;
Lattuada, M;
(2017)
Removal of Cells from Body Fluids by Magnetic Separation in Batch and Continuous Mode: Influence of Bead Size, Concentration, and Contact Time.
ACS Applied Materials & Interfaces
, 9
(35)
pp. 29571-29579.
10.1021/acsami.7b10140.
Preview |
Text
Bertazzo_HerrmannIKetal_R1_clean.pdf - Accepted Version Download (1MB) | Preview |
Abstract
The magnetic separation of pathogenic compounds from body fluids is an appealing therapeutic concept. Recently, removal of a diverse array of pathogens has been demonstrated using extracorporeal dialysis-type devices. The contact time between the fluid and the magnetic beads in such devices is limited to a few minutes. This poses challenges, particularly if large compounds such as bacteria or cells need to be removed. Here, we report on the feasibility to remove cells from body fluids in a continuous dialysis type of setting. We assessed tumor cell removal efficiencies from physiological fluids with or without white blood cells using a range of different magnetic bead sizes (50–4000 nm), concentrations, and contact times. We show that tumor cells can be quantitatively removed from body fluids within acceptable times (1–2 min) and bead concentrations (0.2 mg per mL). We further present a mathematical model to describe the minimal bead number concentration needed to remove a certain number of cells, in the presence of competing nonspecific uptake. The present study paves the way for investigational studies to assess the therapeutic potential of cell removal by magnetic blood purification in a dialysis-like setting.
| Type: | Article |
|---|---|
| Title: | Removal of Cells from Body Fluids by Magnetic Separation in Batch and Continuous Mode: Influence of Bead Size, Concentration, and Contact Time |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acsami.7b10140 |
| Publisher version: | http://doi.org/10.1021/acsami.7b10140 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | blood cells; blood purification; magnetic particles; mathematical modeling; nanoparticles; tumor cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10046918 |
Archive Staff Only
 |
View Item |
