Brunker, J;
Yao, J;
Laufer, J;
Bohndiek, SE;
(2017)
Photoacoustic imaging using genetically encoded reporters: a review.
Journal of Biomedical Optics
, 22
(7)
, Article 070901. 10.1117/1.JBO.22.7.070901.
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Abstract
Genetically encoded contrast in photoacoustic imaging (PAI) is complementary to the intrinsic contrast provided by endogenous absorbing chromophores such as hemoglobin. The use of reporter genes expressing absorbing proteins opens the possibility of visualizing dynamic cellular and molecular processes. This is an enticing prospect but brings with it challenges and limitations associated with generating and detecting different types of reporters. The purpose of this review is to compare existing PAI reporters and signal detection strategies, thereby offering a practical guide, particularly for the nonbiologist, to choosing the most appropriate reporter for maximum sensitivity in the biological and technological system of interest.
| Type: | Article |
|---|---|
| Title: | Photoacoustic imaging using genetically encoded reporters: a review |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1117/1.JBO.22.7.070901 |
| Publisher version: | http://dx.doi.org/10.1117/1.JBO.22.7.070901 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Optics, Radiology, Nuclear Medicine & Medical Imaging, Biochemistry & Molecular Biology, genetic reporters, in vivo, photoacoustics, MULTISPECTRAL OPTOACOUSTIC TOMOGRAPHY, INFRARED FLUORESCENT PROTEINS, IN-VIVO, BACTERIAL PHYTOCHROMES, BLUE CHROMOPROTEIN, OPTICAL-ABSORPTION, OXYGEN-SATURATION, RED, CHROMOPHORE, GENE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10046722 |
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