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Production of a fluorescence resonance energy transfer (FRET) biosensor membrane for microRNA detection

Fu, Y; Chen, T; Wang, G; Gu, T; Xie, C; Huang, J; Li, X; ... Han, G; + view all (2017) Production of a fluorescence resonance energy transfer (FRET) biosensor membrane for microRNA detection. Journal of Materials Chemistry B , 5 (34) pp. 7133-7139. 10.1039/c7tb01399j. Green open access

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Abstract

MicroRNAs (miRNAs) play a key role in regulating gene expression but can be associated with abnormalities linked to carcinogenesis and tumor progression. Hence there is increasing interest in developing methods to detect these noncoding RNA molecules in the human circulation system. Here, a novel FRET miRNA-195 targeting biosensor, based on silica nanofibers incorporated with rare earth-doped calcium fluoride particles (CaF2:Yb,Ho@SiO2) and gold nanoparticles (AuNPs), is reported. The formation of a sandwich structure, as a result of co-hybridization of the target miRNA which is captured by oligonucleotides conjugated at the surface of CaF2:Yb,Ho@SiO2 fibers and AuNPs, brings the nanofibers and AuNPs into close proximity and triggers the FRET effect. The intensity ratio of green to red emission, I541/I650, was found to decrease linearly with increasing concentration of the target miRNA and this can be utilized as a standard curve for quantitative determination of miRNA concentration. This assay offers a simple and convenient method for miRNA quantification, with the potential for rapid and early clinical diagnosis of diseases such as breast cancer.

Type: Article
Title: Production of a fluorescence resonance energy transfer (FRET) biosensor membrane for microRNA detection
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c7tb01399j
Publisher version: http://doi.org/10.1039/c7tb01399j
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10046602
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