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Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development

Alkobtawi, M; Ray, H; Barriga, EH; Moreno, M; Kerney, R; Monsoro-Burq, A-H; Saint-Jeannet, J-P; (2018) Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development. Developmental Biology 10.1016/j.ydbio.2018.02.020. (In press). Green open access

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Abstract

The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transgenic Xenopus laevis lines, Pax3-GFP and Sox10-GFP, in which GFP is expressed in the pre-migratory and migratory neural crest, respectively. We show that Pax3-GFP could be a powerful tool to study neural crest induction, whereas Sox10-GFP could be used in the study of neural crest migration in living embryos.

Type: Article
Title: Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ydbio.2018.02.020
Publisher version: https://doi.org/10.1016/j.ydbio.2018.02.020
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Neural crest, Pax3, Sox10, induction, migration, transgenic
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10045744
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