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Immunohistochemical characterization of feline lymphoplasmacytic anterior uveitis

Crossfield, L; Fortuna, L; Carling, R; Scurrell, E; Matas Riera, M; Chang, Y-M; Dawson, C; ... Garden, OA; + view all (2019) Immunohistochemical characterization of feline lymphoplasmacytic anterior uveitis. Veterinary Ophthalmology , 22 (2) pp. 206-212. 10.1111/vop.12569. Green open access

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Abstract

OBJECTIVE: To characterize the immune cells present in different forms of feline anterior uveitis. SAMPLES: Eyes were obtained from 49 cats diagnosed with chronic idiopathic lymphoplasmacytic anterior uveitis, 7 cats with feline infectious peritonitis (FIP), and 9 cats euthanized for nonocular disease. METHODS: H&E sections were scored on the level of infiltrate in the anterior uvea. Immunohistochemistry was performed for FoxP3, CD3, and IL-17A, and positive cells were quantified in multiple images of each sample. A generalized estimating equation tested for an association between the level of inflammation and the prevalence of these cell types. RESULTS: Cells stained positive for IL-17A in idiopathic uveitis but not in FIP samples. We found significantly fewer FoxP3+and CD3+cells in low-grade compared with high-grade inflammation in idiopathic uveitis or FIP samples (P values all <.005), but no difference between FIP and high-grade samples. CONCLUSIONS: Idiopathic, but not FIP-associated, uveitis appears to have Th17 cell involvement. The numbers of FoxP3+and CD3+T-cells present appear directly correlated; thus, the severity of disease does not appear directly determined by the numbers of regulatory cells.

Type: Article
Title: Immunohistochemical characterization of feline lymphoplasmacytic anterior uveitis
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/vop.12569
Publisher version: https://doi.org/10.1111/vop.12569
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: CD3, FoxP3, IL17, feline, immunohistochemistry, uveitis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10045518
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