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Rap1, canoe and Mbt cooperate with Bazooka to promotezonula adherensassembly in the fly photoreceptor

Walther, RF; Burki, M; Pinal, N; Rogerson, C; Pichaud, F; (2018) Rap1, canoe and Mbt cooperate with Bazooka to promotezonula adherensassembly in the fly photoreceptor. Journal of Cell Science , 131 (6) , Article jcs207779. 10.1242/jcs.207779. Green open access

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Abstract

In Drosophila epithelial cells, apical exclusion of Bazooka/Par3 defines the position of the Zonula Adherens (ZA), which demarcates the apical and lateral membrane and allows cells to assemble into sheets. Here, we show that the small GTPase Rap1, its effector AF6/Canoe (Cno) and the Cdc42-effector Pak4/Mushroom bodies tiny (Mbt), converge in regulating epithelial morphogenesis by coupling stabilization of the Adherens Junction (AJ) protein E-Cadherin, and Bazooka retention at the ZA. Furthermore, our results show that the localization of Rap1, Cno and Mbt at the ZA is interdependent, indicating their functions during ZA morphogenesis are interlinked. In this context, we find the Rap1-GEF Dizzy is enriched at the ZA and our results suggest it promotes Rap1 activity during ZA morphogenesis. Altogether, we propose the Dizzy, Rap1/Cno pathway and Mbt converge in regulating the interface between Bazooka and AJ material to promote ZA morphogenesis.

Type: Article
Title: Rap1, canoe and Mbt cooperate with Bazooka to promotezonula adherensassembly in the fly photoreceptor
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.207779
Publisher version: http://jcs.biologists.org/content/early/2018/02/14...
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Epithelial polarity, Pak4, Par3/Bazooka, Photoreceptor, Rap1, Zonula adherens
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10045504
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