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A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi

Bottanelli, F; Kilian, N; Ernst, AM; Rivera-Molina, F; Schroeder, LK; Kromann, EB; Lessard, MD; ... Rothman, JE; + view all (2017) A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi. Molecular Biology of the Cell , 28 (12) pp. 1676-1687. 10.1091/mbc.E16-12-0863. Green open access

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Abstract

Capitalizing on CRISPR/Cas9 gene-editing techniques and super-resolution nanoscopy, we explore the role of the small GTPase ARF1 in mediating transport steps at the Golgi. Besides its well-established role in generating COPI vesicles, we find that ARF1 is also involved in the formation of long (∼3 µm), thin (∼110 nm diameter) tubular carriers. The anterograde and retrograde tubular carriers are both largely free of the classical Golgi coat proteins coatomer (COPI) and clathrin. Instead, they contain ARF1 along their entire length at a density estimated to be in the range of close packing. Experiments using a mutant form of ARF1 affecting GTP hydrolysis suggest that ARF1[GTP] is functionally required for the tubules to form. Dynamic confocal and stimulated emission depletion imaging shows that ARF1-rich tubular compartments fall into two distinct classes containing 1) anterograde cargoes and clathrin clusters or 2) retrograde cargoes and coatomer clusters.

Type: Article
Title: A novel physiological role for ARF1 in the formation of bidirectional tubules from the Golgi
Open access status: An open access version is available from UCL Discovery
DOI: 10.1091/mbc.E16-12-0863
Publisher version: http://dx.doi.org/10.1091/mbc.E16-12-0863
Language: English
Additional information: Copyright © 2017 Bottanelli et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0)
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy
URI: https://discovery.ucl.ac.uk/id/eprint/10044874
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