Collins, A;
Li, D;
Liadi, M;
Tabakow, P;
Fortuna, W;
Raisman, G;
Li, Y;
(2018)
Partial recovery of proprioception in rats with dorsal root injury following human olfactory bulb cell transplantation.
Journal of Neurotrauma
, 25
(12)
pp. 1367-1378.
10.1089/neu.2017.5273.
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Abstract
We transplanted human olfactory bulb OECs (hOECs) mixed with collagen into a unilateral transection of four dorsal roots (C6-T1) in a rat model. By mixing with collagen, we could maximise the limited numbers of hOEC from an olfactory bulb biopsy and optimise cavity-filling. Cyclosporine was administered daily to prevent immune rejection. Forelimb proprioception was assessed weekly in a vertical climb task. Half of the rats receiving hOEC transplants showed some functional improvement ('responders') over six weeks of the study whilst the other half did not ('non-responders') and performed similarly to 'injured only' rats. Transplanted cells were seen at both 1 week and 6 weeks after surgery; many were concentrated within the lesion cavity but others were found with elongated processes in the overlying connective tissue. There were some fibres in the injury area associated with transplanted cells which were immunostained for neurofilament and TUJ1. We compared responder and non-responder rats with regard to microglial activation within the deep dorsal horn of cervical levels C7,C8 and also axon loss within the cuneate fasciculus at cervical level C3. We saw little difference in microglial activation or axonal loss which could account for the improved proprioception in the 'responders' group. We believe this preliminary study is the first to transplant human olfactory bulb cells into a rat model of dorsal root injury; by refining each component part of the procedure, we can continue to maximise the repair potential of OECs in a clinical setting. Key words: spinal roots, glia, rhizotomy, translational model.
| Type: | Article |
|---|---|
| Title: | Partial recovery of proprioception in rats with dorsal root injury following human olfactory bulb cell transplantation |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1089/neu.2017.5273 |
| Publisher version: | https://doi.org/10.1089/neu.2017.5273 |
| Language: | English |
| Additional information: | © Mary Ann Liebert, Inc. 2017. This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | TRANSPLANTATION, axonal regeneration, spinal cord injury |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Brain Repair and Rehabilitation |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10042480 |
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