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Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model.

Mohmmed, SA; Vianna, ME; Penny, MR; Hilton, ST; Mordan, NJ; Knowles, JC; (2018) Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model. International Endodontic Journal , 51 (6) pp. 649-662. 10.1111/iej.12880. Green open access

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Abstract

AIM: To investigate in situ Enterococcus faecalis biofilm removal from the lateral canal of a simulated root canal system using passive or active irrigation protocols. METHODOLOGY: Root canal models (n = 43) were manufactured from transparent resin materials using 3D-printing. Each canal was created with an 18 mm length, apical size 30, a .06 taper, and a lateral canal of 3 mm length, 0.3 mm diameter. Biofilms were grown on the lateral canal and apical 3 mm of the main canal for 10 days. Biofilm of three models was examined using SEM. The other forty models were divided to four groups (n = 10). The models were observed under a fluorescence microscope. Following 60 s of 9 mL of 2.5% NaOCl irrigation using syringe and needle, the irrigant was either left stagnant in the canal or activated using gutta-percha, sonic or ultrasonic methods for 30 s. Images were then captured every second using an external camera. The residual biofilm percentages were measured using image analysis software. The data were analyzed using generalized linear mixed models. A significance level of 0.05 was used throughout. RESULTS: The greatest level of biofilm removal was with ultrasonic agitation (66.76%) followed by sonic (45.49%), manual agitation (43.97%), and passive irrigation groups (38.67%) respectively. The differences were significant between the residual biofilm in the passive irrigation and both sonic & ultrasonic groups (P = 0.001). CONCLUSION: Agitation resulted in better penetration of the 2.5% NaOCl into the lateral canal of an artificial root canal model. Ultrasonic agitation of NaOCl improved the removal of biofilm. This article is protected by copyright. All rights reserved.

Type: Article
Title: Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model.
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/iej.12880
Publisher version: http://dx.doi.org/10.1111/iej.12880
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: 3D printing model, Automated agitation, Enterococcus faecalis biofilm, lateral canal, manual agitation, sodium hypochlorite
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10039021
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