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Optimized Lateral Flow Immunoassay Reader for the Detection of Infectious Diseases in Developing Countries

Pilavaki, E; Demosthenous, A; (2017) Optimized Lateral Flow Immunoassay Reader for the Detection of Infectious Diseases in Developing Countries. Sensors , 17 (11) , Article 2673. 10.3390/s17112673. Green open access

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Abstract

Detection and control of infectious diseases is a major problem, especially in developing countries. Lateral flow immunoassays can be used with great success for the detection of infectious diseases. However, for the quantification of their results an electronic reader is required. This paper presents an optimized handheld electronic reader for developing countries. It features a potentially low-cost, low-power, battery-operated device with no added optical accessories. The operation of this proof of concept device is based on measuring the reflected light from the lateral flow immunoassay and translating it into the concentration of the specific analyte of interest. Characterization of the surface of the lateral flow immunoassay has been performed in order to accurately model its response to the incident light. Ray trace simulations have been performed to optimize the system and achieve maximum sensitivity by placing all the components in optimum positions. A microcontroller enables all the signal processing to be performed on the device and a Bluetooth module allows transmission of the results wirelessly to a mobile phone app. Its performance has been validated using lateral flow immunoassays with influenza A nucleoprotein in the concentration range of 0.5 ng/mL to 200 ng/mL.

Type: Article
Title: Optimized Lateral Flow Immunoassay Reader for the Detection of Infectious Diseases in Developing Countries
Location: Switzerland
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/s17112673
Publisher version: http://dx.doi.org/10.3390/s17112673
Language: English
Additional information: Copyright © 2017 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: infectious diseases, lateral flow immunoassay, point of care, ray trace simulation, reader
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10038750
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