Rattazzi, L;
Piras, G;
Brod, S;
Smith, K;
Ono, M;
D'Acquisto, F;
(2016)
Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile.
Frontiers in Immunology
, 7
, Article 381. 10.3389/fimmu.2016.00381.
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Abstract
T cells are known to be plastic and to change their phenotype according to the cellular and biochemical milieu they are embedded in. In this study, we transposed this concept at a macroscopic level assessing whether changes in the environmental housing conditions of C57/BL6 mice would influence the phenotype and function of T cells. Our study shows that exposure to 2 weeks in an enriched environment (EE) does not impact the T cell repertoire in vivo and causes no changes in the early TCR-driven activation events of these cells. Surprisingly, however, T cells from enriched mice showed a unique T helper effector cell phenotype upon differentiation in vitro. This was featured by a significant reduction in their ability to produce IFN-γ and by an increased release of IL-10 and IL-17. Microarray analysis of these cells also revealed a unique gene fingerprint with key signaling pathways involved in autoimmunity being modulated. Together, our results provide first evidence for a specific effect of EE on T cell differentiation and its associated changes in gene expression profile. In addition, our study sheds new light on the possible mechanisms by which changes in environmental factors can significantly influence the immune response of the host and favor the resolution of the inflammatory response.
| Type: | Article |
|---|---|
| Title: | Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fimmu.2016.00381 |
| Publisher version: | http://dx.doi.org/10.3389/fimmu.2016.00381 |
| Language: | English |
| Additional information: | © 2016 Rattazzi, Piras, Brod, Smith, Ono and D’Acquisto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Immunology, CD4 T cells, enriched environment, microarray, cytokines, resolution of inflammation, EXPERIENCE-DEPENDENT PLASTICITY, TH17 CELLS, AUTOIMMUNE-DISEASES, PHYSICAL-ACTIVITY, IMMUNE-RESPONSES, TRANSGENIC MICE, ADULT MICE, BRAIN, ACTIVATION, MODEL |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10038706 |
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