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Development of a 3D collagen model for the in vitro evaluation of magnetic stimulation on osteogenesis

Yuan, Rebecca Zhiyu; (2017) Development of a 3D collagen model for the in vitro evaluation of magnetic stimulation on osteogenesis. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

Magnetic stimulation has been applied to bone regeneration and fracture non-union treatments, however, the cellular and molecular mechanisms of repair still require better understanding. In this study, a three-dimensional (3D) collagen model has been developed using plastic compression (PC), which produces dense, cellular, and mechanically strong native collagen structures. Osteoblast cells (MG-63, UMR-106, MC3T3-E1), bioactive nano-hydroxyapatite (nHA) and magnetic iron oxide nanoparticles (IONPs), were incorporated into the collagen gels to produce a range of cell-laden models. A magnetic bio-reactor to support cell growth under static magnetic fields (SMFs) was designed and fabricated by 3D printing. The influences of SMFs on cell proliferation, differentiation, extracellular matrix production, mineralisation and gene expression were evaluated. Results demonstrated that SMFs and IONPs stimulated the proliferation, alkaline phosphatase (ALP) production and level of mineralisation of MG-63 cells in vitro. Transmission Electron Microscopy (TEM) examination showed some changes in microstructure of collagen fibres subjected to SMFs. Real-time polymerase chain reaction (PCR) investigation further determined the effects of SMFs on the expression of Runtrelated transcription factor 2 (Runx2), osteonectin (ON), and bone morphogenic protein 2 and 4 (BMP-2 and BMP-4). The stimulating effects were identified as the combination of SMFs and IONPs, which can enhance the osteogenesis process in vitro. The results indicated that the magnetic stimulation influences the matrix/cell interactions, and is capable of encouraging gene expression. Therefore, the collagen model developed in this study not only offers a novel 3D bone model to better understand the effects of magnetic stimulation on osteogenesis, but also paves the way for further applications in tissue engineering and regenerative medicine.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Development of a 3D collagen model for the in vitro evaluation of magnetic stimulation on osteogenesis
Event: UCL (University College London)
Open access status: An open access version is available from UCL Discovery
Language: English
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
URI: https://discovery.ucl.ac.uk/id/eprint/10038296
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