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An L319F mutation in transmembrane region 3 (TM3) selectively reduces sensitivity to okaramine B of the Bombyx mori L-glutamate-gated chloride channel

Furutani, S; Okuhara, D; Hashimoto, A; Ihara, M; Kai, K; Hayashi, H; Sattelle, DB; (2017) An L319F mutation in transmembrane region 3 (TM3) selectively reduces sensitivity to okaramine B of the Bombyx mori L-glutamate-gated chloride channel. Bioscience, Biotechnology, and Biochemistry , 81 (10) pp. 1861-1867. 10.1080/09168451.2017.1359487. Green open access

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Abstract

Okaramines produced by Penicillium simplicissimum AK-40 activate l-glutamate-gated chloride channels (GluCls) and thus paralyze insects. However, the okaramine binding site on insect GluCls is poorly understood. Sequence alignment shows that the equivalent of residue Leucine319 of the okaramine B sensitive Bombyx mori (B. mori) GluCl is a phenylalanine in the okaramine B insensitive B. mori γ-aminobutyric acid-gated chloride channel of the same species. This residue is located in the third transmembrane (TM3) region, a location which in a nematode GluCl is close to the ivermectin binding site. The B. mori GluCl containing the L319F mutation retained its sensitivity to l-glutamate, but responses to ivermectin were reduced and those to okaramine B were completely blocked.

Type: Article
Title: An L319F mutation in transmembrane region 3 (TM3) selectively reduces sensitivity to okaramine B of the Bombyx mori L-glutamate-gated chloride channel
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/09168451.2017.1359487
Publisher version: http://doi.org/10.1080/09168451.2017.1359487
Language: English
Additional information: © 2017 Japan Society for Bioscience, Biotechnology, and Agrochemistry. This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemistry & Molecular Biology, Biotechnology & Applied Microbiology, Chemistry, Applied, Food Science & Technology, Chemistry, okaramine, L-glutamate-gated chloride channel, ion channel, ivermectin, Bombyx mori, NICOTINIC ACETYLCHOLINE-RECEPTOR, NEONICOTINOID INSECTICIDES, LOOP-D, ALKALOIDS, SILKWORM, RESIDUES, ALPHA-7
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10035885
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