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miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition

Beclin, C; Follert, P; Stappers, E; Barral, S; Nathalie, C; de Chevigny, A; Magnone, V; ... Cremer, H; + view all (2016) miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition. SCIENTIFIC REPORTS , 6 , Article 357. 10.1038/srep35729. Green open access

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Abstract

During neurogenesis, generation, migration and integration of the correct numbers of each neuron sub-type depends on complex molecular interactions in space and time. MicroRNAs represent a key control level allowing the flexibility and stability needed for this process. Insight into the role of this regulatory pathway in the brain is still limited. We performed a sequential experimental approach using postnatal olfactory bulb neurogenesis in mice, starting from global expression analyses to the investigation of functional interactions between defined microRNAs and their targets. Deep sequencing of small RNAs extracted from defined compartments of the postnatal neurogenic system demonstrated that the miR-200 family is specifically induced during late neuronal differentiation stages. Using in vivo strategies we interfered with the entire miR-200 family in loss- and gain-of-function settings, showing a role of miR-200 in neuronal maturation. This function is mediated by targeting the transcription factor Zeb2. Interestingly, so far functional interaction between miR-200 and Zeb2 has been exclusively reported in cancer or cultured stem cells. Our data demonstrate that this regulatory interaction is also active during normal neurogenesis.

Type: Article
Title: miR-200 family controls late steps of postnatal forebrain neurogenesis via Zeb2 inhibition
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/srep35729
Publisher version: http://dx.doi.org/10.1038/srep35729
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, EPITHELIAL-MESENCHYMAL TRANSITION, ADULT SUBVENTRICULAR ZONE, OLFACTORY-BULB, BRAIN-DEVELOPMENT, E-CADHERIN, DOPAMINERGIC-NEURONS, MOTILE CILIOGENESIS, REPRESSORS ZEB1, FEEDBACK LOOP, CANCER-CELLS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Neurosciences Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10033933
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