Filer, D; Thompson, MA; Takhaveev, V; Dobson, AJ; Kotranaki, I; Green, JWM; Heinemann, M; ... Alic, N; + view all Filer, D; Thompson, MA; Takhaveev, V; Dobson, AJ; Kotranaki, I; Green, JWM; Heinemann, M; Tullet, JMA; Alic, N; - view fewer (2017) RNA polymerase III limits longevity downstream of TORC1. Nature , 552 pp. 263-267. 10.1038/nature25007.

Preview	Text Filer_etal.pdf - Accepted Version Download (9MB) | Preview
	Spreadsheet EDFig1DATA.xlsx - Accepted Version Download (127kB)
	Spreadsheet EDFig2DATA.xlsx - Accepted Version Download (39kB)
	Spreadsheet EDFig4DATA.xlsx - Accepted Version Download (61kB)
	Spreadsheet EDFig6DATA.xlsx - Accepted Version Download (64kB)
	Spreadsheet EDFig7DATA.xlsx - Accepted Version Download (48kB)
	Spreadsheet EDFig8DATA.xlsx - Accepted Version Download (40kB)
	Spreadsheet EDFig9DATA.xlsx - Accepted Version Download (42kB)
	Spreadsheet Fig1DATA.xlsx - Accepted Version Download (44kB)
	Spreadsheet Fig2DATA.xlsx - Accepted Version Download (53kB)
	Spreadsheet Fig3DATA.xlsx - Accepted Version Download (49kB)
	Spreadsheet Fig4DATA.xlsx - Accepted Version Download (35kB)

Abstract

Three distinct RNA polymerases transcribe different classes of genes in the eukaryotic nucleus. RNA polymerase (Pol) III is the essential, evolutionarily conserved enzyme that generates short, non-coding RNAs, including tRNAs and 5S rRNA. The historical focus on transcription of protein-coding genes has left the roles of Pol III in organismal physiology relatively unexplored. Target of rapamycin kinase complex 1 (TORC1) regulates Pol III activity, and is also an important determinant of longevity. This raises the possibility that Pol III is involved in ageing. Here we show that Pol III limits lifespan downstream of TORC1. We find that a reduction in Pol III extends chronological lifespan in yeast and organismal lifespan in worms and flies. Inhibiting the activity of Pol III in the gut of adult worms or flies is sufficient to extend lifespan; in flies, longevity can be achieved by Pol III inhibition specifically in intestinal stem cells. The longevity phenotype is associated with amelioration of age-related gut pathology and functional decline, dampened protein synthesis and increased tolerance of proteostatic stress. Pol III acts on lifespan downstream of TORC1, and limiting Pol III activity in the adult gut achieves the full longevity benefit of systemic TORC1 inhibition. Hence, Pol III is a pivotal mediator of this key nutrient-signalling network for longevity; the growth-promoting anabolic activity of Pol III mediates the acceleration of ageing by TORC1. The evolutionary conservation of Pol III affirms its potential as a therapeutic target.

Download activity - last month

Export as XMLCSVJSON

Download activity - last 12 months

Export as JSONCSVXML

Downloads by country - last 12 months

Export as XMLCSVJSON

Archive Staff Only

View Item