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Patch method for culture of primary hippocampal neurons

Tang, Y; Severino, FPU; Iseppon, F; Torre, V; Chen, Y; (2017) Patch method for culture of primary hippocampal neurons. Microelectronic Engineering , 175 pp. 61-66. 10.1016/j.mee.2017.01.012. Green open access

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Abstract

Culture of primary neurons, and especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method for culture of primary neurons on a monolayer of gelatin nanofibers electrospun and crosslinked on a honeycomb microframe of poly (ethylene glycol) diacrylate (PEGDA). This method allows us to minimize exogenous material contact of cells and largely increase the exposure area of cells to the culture medium. We found that neurons, and especially astrocytes, have a more in vivo like morphology comparing to that on culture dish or on glass slide. We also found that neurons were preferentially located in the suspended areas of the monolayer nanofibers. Finally, calcium imaging revealed that primary neurons have a higher degree of neural activity on the patch than on glass. These results suggest that crosslinked and monolayer gelatin nanofibers closely mimic the extracellular matrix structure and allow more effective culture of primary neurons than conventional methods, thus facilitating advanced studies of neural functions as well as cell-based assays.

Type: Article
Title: Patch method for culture of primary hippocampal neurons
Event: 42nd Conference on Micro- and Nano-Engineering (MNE)
Location: Vienna, AUSTRIA
Dates: 19 September 2016 - 23 September 2016
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.mee.2017.01.012
Publisher version: http://doi.org/10.1016/j.mee.2017.01.012
Language: English
Additional information: © 2017 Published by Elsevier B.V. This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Nanofiber, Patch, Hippocampal neuro, Calcium imaging
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research
URI: https://discovery.ucl.ac.uk/id/eprint/10033078
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