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Single cell genomics of uncultured marine alveolates shows paraphyly of basal dinoflagellates

Strassert, JFH; Karnkowska, A; Hehenberger, E; del Campo, J; Kolisko, M; Okamoto, N; Burki, F; ... Keeling, PJ; + view all (2018) Single cell genomics of uncultured marine alveolates shows paraphyly of basal dinoflagellates. The ISME Journal , 12 pp. 304-308. 10.1038/ismej.2017.167. Green open access

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Abstract

Marine alveolates (MALVs) are diverse and widespread early-branching dinoflagellates, but most knowledge of the group comes from a few cultured species that are generally not abundant in natural samples, or from diversity analyses of PCR-based environmental SSU rRNA gene sequences. To more broadly examine MALV genomes, we generated single cell genome sequences from seven individually isolated cells. Genes expected of heterotrophic eukaryotes were found, with interesting exceptions like presence of proteorhodopsin and vacuolar H+-pyrophosphatase. Phylogenetic analysis of concatenated SSU and LSU rRNA gene sequences provided strong support for the paraphyly of MALV lineages. Dinoflagellate viral nucleoproteins were found only in MALV groups that branched as sister to dinokaryotes. Our findings indicate that multiple independent origins of several characteristics early in dinoflagellate evolution, such as a parasitic life style, underlie the environmental diversity of MALVs, and suggest they have more varied trophic modes than previously thought.

Type: Article
Title: Single cell genomics of uncultured marine alveolates shows paraphyly of basal dinoflagellates
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/ismej.2017.167
Publisher version: http://dx.doi.org/10.1038/ismej.2017.167
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10030027
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