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High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process optimization

Velez-Suberbie, ML; Betts, JPJ; Walker, KL; Robinson, C; Zoro, B; Keshavarz-Moore, E; (2018) High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process optimization. Biotechnology Progress , 34 (1) pp. 58-68. 10.1002/btpr.2534. Green open access

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Abstract

High throughput automated fermentation systems have become a useful tool in early bioprocess development. In this study, we investigated a 24 x 15 mL single use microbioreactor system, ambr 15f, designed for microbial culture. We compared the fed-batch growth and production capabilities of this system for two Escherichia coli strains, BL21 (DE3) and MC4100, and two industrially relevant molecules, hGH and scFv. In addition, different carbon sources were tested using bolus, linear or exponential feeding strategies, showing the capacity of the ambr 15f system to handle automated feeding. We used power per unit volume (P/V) as a scale criterion to compare the ambr 15f with 1 L stirred bioreactors which were previously scaled-up to 20 L with a different biological system, thus showing a potential 1,300 fold scale comparability in terms of both growth and product yield. By exposing the cells grown in the ambr 15f system to a level of shear expected in an industrial centrifuge, we determined that the cells are as robust as those from a bench scale bioreactor. These results provide evidence that the ambr 15f system is an efficient high throughput microbial system that can be used for strain and molecule selection as well as rapid scale-up. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 2017.

Type: Article
Title: High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process optimization
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/btpr.2534
Publisher version: http://doi.org/10.1002/btpr.2534
Language: English
Additional information: © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: E. coli, ambr 15 fermentation, high throughput, scale-down, single use microbioreactor
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10025208
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