eprintid: 96111 rev_number: 79 eprint_status: archive userid: 608 dir: disk0/00/09/61/11 datestamp: 2010-10-19 10:29:37 lastmod: 2022-01-24 23:28:55 status_changed: 2012-07-11 16:00:38 type: article metadata_visibility: show item_issues_count: 0 creators_name: Yu, CK creators_name: Casas, JP creators_name: Savvidou, MD creators_name: Sahemey, MK creators_name: Nicolaides, KH creators_name: Hingorani, AD title: Endothelial nitric oxide synthase gene polymorphism (Glu298Asp) and development of pre-eclampsia: a case-control study and a meta-analysis. ispublished: pub divisions: UCL divisions: B02 divisions: D14 divisions: DD4 note: © 2006 Yu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. abstract: Background: Pre-eclampsia is thought to have an important genetic component. Recently, pre-eclampsia has been associated in some studies with carriage of a common eNOS gene Glu298Asp polymorphism, a variant that leads to the replacement of glutamic acid by aspartic acid at codon 298. Method: Healthy women with singleton pregnancies were recruited from 7 district general hospitals in London, UK. Women at high risk of pre-eclampsia were screened by uterine artery Doppler velocimetry at 22–24 weeks of gestation and maternal blood was obtained to genotype the eNOS Glu298Asp polymorphism. Odds ratios (OR) and 95%CI, using logistic regression methods, were obtained to evaluate the association between the Glu298Asp polymorphism and pre-eclampsia. A meta-analysis was then undertaken of all published studies up to November 2005 examining the association of eNOS Glu298Asp genotype and pre-eclampsia. Results: 89 women with pre-eclampsia and 349 controls were included in the new study. The Glu298Asp polymorphism in a recessive model was not significantly associated with pre-eclampsia (adjusted-OR: 0.83 [95%CI: 0.30–2.25]; p = 0.7). In the meta-analysis, under a recessive genetic model (1129 cases & 2384 controls) women homozygous for the Asp298 allele were not at significantly increased risk of pre-eclampsia (OR: 1.28 [95%CI: 0.76–2.16]; p = 0.34). A dominant model (1334 cases & 2894 controls) was associated with no increase of risk of pre-eclampsia for women carriers of the Asp298 allele (OR: 1.12 [95%CI: 0.84–1.49]; p = 0.42). Conclusion: From the data currently available, the eNOS Glu298Asp polymorphism is not associated with a significant increased risk of pre-eclampsia. However, published studies have been underpowered, much larger studies are needed to confirm or refute a realistic genotypic risk of disease, but which might contribute to many cases of pre-eclampsia in the population. date: 2006-03-16 official_url: http://dx.doi.org/10.1186/1471-2393-6-7 vfaculties: VFPHS oa_status: green language: eng primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_source: PubMed elements_id: 169203 doi: 10.1186/1471-2393-6-7 pii: 1471-2393-6-7 lyricists_name: Casas Romero, Juan lyricists_name: Hingorani, Aroon lyricists_id: JPCAS59 lyricists_id: AHING65 full_text_status: public publication: BMC Pregnancy and Childbirth volume: 6 article_number: 7 pagerange: - event_location: England citation: Yu, CK; Casas, JP; Savvidou, MD; Sahemey, MK; Nicolaides, KH; Hingorani, AD; (2006) Endothelial nitric oxide synthase gene polymorphism (Glu298Asp) and development of pre-eclampsia: a case-control study and a meta-analysis. BMC Pregnancy and Childbirth , 6 , Article 7. 10.1186/1471-2393-6-7 <https://doi.org/10.1186/1471-2393-6-7>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/96111/1/1471-2393-6-7.pdf