eprintid: 7831 rev_number: 67 eprint_status: archive userid: 599 dir: disk0/00/00/78/31 datestamp: 2008-10-02 15:17:22 lastmod: 2021-12-31 22:32:12 status_changed: 2008-10-02 15:17:22 type: article metadata_visibility: show item_issues_count: 0 creators_name: Lambiase, PD creators_name: Edwards, RJ creators_name: Anthopoulos, P creators_name: Rahman, S creators_name: Meng, YG creators_name: Bucknall, CA creators_name: Redwood, SR creators_name: Pearson, JD creators_name: Marber, MS title: Circulating humoral factors and endothelial progenitor cells in patients with differing coronary collateral support subjects: 4300 divisions: UCL divisions: B02 divisions: D14 divisions: GA4 keywords: collateral circulation, angiogenesis, coronary disease, ARTERY-DISEASE, BLOOD-FLOW, POSTNATAL NEOVASCULARIZATION, ANGIOGENESIS, GROWTH, TRANSPLANTATION, EXPRESSION, INCREASE, PATHWAYS, THERAPY abstract: Background - The mechanisms underlying the variation in collateral formation between patients, even with similar patterns of coronary artery disease, remain unclear. This study investigates whether circulating humoral or cellular factors can provide an insight into this variation.Methods and Results - Thirty patients with isolated left anterior descending coronary artery disease underwent percutaneous coronary intervention with collateral flow index (CFI) determined using a pressure wire. Patients with inadequate ( CFI < 0.25) compared with those with adequate ( CFI &GE; 0.25) collateral support had, or tended to have, lower concentrations of coronary sinus growth factors and plasma exerting a weaker effect on endothelial cell migration and angiogenesis in vitro. However, there was an inverse correlation between serum mitogenicity and CFI ( r = - 0.61, P < 0.01). No significant differences were detected between the 2 groups in plasma levels of total vascular endothelial growth factor, vascular endothelial growth factor(165), or placental growth factor. There was a strong positive correlation between numbers of CD34/CD133-positive circulating hemopoietic precursor cells and CFI ( r = 0.75, P < 0.001). In patients with inadequate, compared with those with adequate, CFI, the numbers of differentiated endothelial progenitor cells (EPCs) appearing in the circulation and in culture were significantly reduced by 75% ( P < 0.05) and 70% ( P < 0.05), respectively.Conclusions - In this study, inadequate coronary collateral development is associated with reduced numbers of circulating EPCs and impaired chemotactic and proangiogenic but not mitogenic activity. These findings are consistent with current efforts to enhance collateral formation by augmentation of circulating EPCs. date: 2004-06-22 date_type: published publisher: LIPPINCOTT WILLIAMS & WILKINS vfaculties: VFPHS rae2008: 4 oa_status: green primo: open primo_central: open_green article_type_text: Article verified: verified_batch elements_source: Web of Science elements_id: 57886 doi: 10.1161/01.CIR.0000130639.97284.EC lyricists_name: Lambiase, David lyricists_id: DLAMB45 full_text_status: public publication: CIRCULATION volume: 109 number: 24 pagerange: 2986 - 2992 refereed: TRUE issn: 0009-7322 citation: Lambiase, PD; Edwards, RJ; Anthopoulos, P; Rahman, S; Meng, YG; Bucknall, CA; Redwood, SR; ... Marber, MS; + view all <#> Lambiase, PD; Edwards, RJ; Anthopoulos, P; Rahman, S; Meng, YG; Bucknall, CA; Redwood, SR; Pearson, JD; Marber, MS; - view fewer <#> (2004) Circulating humoral factors and endothelial progenitor cells in patients with differing coronary collateral support. CIRCULATION , 109 (24) 2986 - 2992. 10.1161/01.CIR.0000130639.97284.EC <https://doi.org/10.1161/01.CIR.0000130639.97284.EC>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/7831/1/7831.pdf