eprintid: 6980
rev_number: 34
eprint_status: archive
userid: 598
dir: disk0/00/00/69/80
datestamp: 2008-09-24 09:13:25
lastmod: 2015-07-23 09:34:09
status_changed: 2008-09-24 09:13:25
type: article
metadata_visibility: show
creators_name: Whalley, S.A.
creators_name: Murray, J.M.
creators_name: Brown, D.
creators_name: Webster, G.J.M.
creators_name: Emery, V.C.
creators_name: Dusheiko, G.M.
creators_name: Perelson, A.S.
creators_id: 
creators_id: 
creators_id: 
creators_id: 
creators_id: VCEME33
creators_id: 
creators_id: 
title: Kinetics of acute hepatitis B virus infection in humans
ispublished: pub
subjects: 3665
keywords: Acute hepatitis B, kinetics, cytotoxic T lymphocytes, mutation, hepatocyte
abstract: Using patient data from a unique single source outbreak of hepatitis B virus (HBV) infection, we have characterized the kinetics of acute HBV infection by monitoring viral turnover in the serum during the late incubation and clinical phases of the disease in humans. HBV replicates rapidly with minimally estimated doubling times ranging between 2.2 and 5.8 d (mean 3.7 ± 1.5 d). After a peak viral load in serum of nearly 1010 HBV DNA copies/ml is attained, clearance of HBV DNA follows a two or three phase decay pattern with an initial rapid decline characterized by mean half-life (t1/2) of 3.7 ± 1.2 d, similar to the t1/2 observed in the noncytolytic clearance of covalently closed circular DNA for other hepadnaviruses. The final phase of virion clearance occurs at a variable rate (t1/2 of 4.8 to 284 d) and may relate to the rate of loss of infected hepatocytes. Free virus has a mean t1/2 of at most 1.2 ± 0.6 d. We estimate a peak HBV production rate of at least 1013 virions/day and a maximum production rate of an infected hepatocyte of 200–1,000 virions/day, on average. At this peak rate of virion production we estimate that every possible single and most double mutations would be created each day.
date: 2001-04-02
date_type: published
official_url: http://dx.doi.org/10.1084/jem.193.7.847
rae2008: 3
oa_status: green
language: eng
primo: open
primo_central: open_green
doi: 10.1084/jem.193.7.847
lyricists_name: Emery, V
lyricists_id: VCEME33
full_text_status: public
publication: Journal of Experimental Medicine
volume: 193
number: 7
pagerange: 847-854
refereed: TRUE
issn: 0022-1007
citation:        Whalley, S.A.;    Murray, J.M.;    Brown, D.;    Webster, G.J.M.;    Emery, V.C.;    Dusheiko, G.M.;    Perelson, A.S.;      (2001)    Kinetics of acute hepatitis B virus infection in humans.                   Journal of Experimental Medicine , 193  (7)   pp. 847-854.    10.1084/jem.193.7.847 <https://doi.org/10.1084/jem.193.7.847>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/6980/1/6980.pdf