eprintid: 358409
rev_number: 43
eprint_status: archive
userid: 608
dir: disk0/00/35/84/09
datestamp: 2010-12-10 14:51:05
lastmod: 2021-12-13 01:56:17
status_changed: 2010-12-10 14:51:05
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Dallosso, AR
creators_name: Hancock, AL
creators_name: Szemes, M
creators_name: Moorwood, K
creators_name: Chilukamarri, L
creators_name: Tsai, HH
creators_name: Sarkar, A
creators_name: Barasch, J
creators_name: Vuononvirta, R
creators_name: Jones, C
creators_name: Pritchard-Jones, K
creators_name: Royer-Pokora, B
creators_name: Lee, SB
creators_name: Owen, C
creators_name: Malik, S
creators_name: Feng, Y
creators_name: Frank, M
creators_name: Ward, A
creators_name: Brown, KW
creators_name: Malik, K
title: Frequent Long-Range Epigenetic Silencing of Protocadherin Gene Clusters on Chromosome 5q31 in Wilms' Tumor
ispublished: pub
divisions: UCL
divisions: B02
divisions: D13
divisions: G22
keywords: GAMMA-PROTOCADHERINS, BREAST-CANCER, COLORECTAL-CANCER, SUPPRESSOR GENE, KIDNEY DEVELOPMENT, NEPHROGENIC RESTS, DNA METHYLATION, COMMON EVENT, LUNG-CANCER, CELL-LINE
note: This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

Funding: This work was supported by Cancer and Leukaemia in Childhood - Sargent, the Children's Leukaemia Trust, and Kidney Research UK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
abstract: Wilms' tumour (WT) is a pediatric tumor of the kidney that arises via failure of the fetal developmental program. The absence of identifiable mutations in the majority of WTs suggests the frequent involvement of epigenetic aberrations in WT. We therefore conducted a genome-wide analysis of promoter hypermethylation in WTs and identified hypermethylation at chromosome 5q31 spanning 800 kilobases (kb) and more than 50 genes. The methylated genes all belong to alpha-, beta-, and gamma-protocadherin (PCDH) gene clusters (Human Genome Organization nomenclature PCDHA@, PCDHB@, and PCDHG@, respectively). This demonstrates that long-range epigenetic silencing (LRES) occurs in developmental tumors as well as in adult tumors. Bisulfite polymerase chain reaction analysis showed that PCDH hypermethylation is a frequent event found in all Wilms' tumor subtypes. Hypermethylation is concordant with reduced PCDH expression in tumors. WT precursor lesions showed no PCDH hypermethylation, suggesting that de novo PCDH hypermethylation occurs during malignant progression. Discrete boundaries of the PCDH domain are delimited by abrupt changes in histone modifications; unmethylated genes flanking the LRES are associated with permissive marks which are absent from methylated genes within the domain. Silenced genes are marked with non-permissive histone 3 lysine 9 dimethylation. Expression analysis of embryonic murine kidney and differentiating rat metanephric mesenchymal cells demonstrates that Pcdh expression is developmentally regulated and that Pcdhg@ genes are expressed in blastemal cells. Importantly, we show that PCDHs negatively regulate canonical Wnt signalling, as short-interfering RNA-induced reduction of PCDHG@ encoded proteins leads to elevated beta-catenin protein, increased beta-catenin/T-cell factor (TCF) reporter activity, and induction of Wnt target genes. Conversely, over-expression of PCDHs suppresses beta-catenin/TCF-reporter activity and also inhibits colony formation and growth of cancer cells in soft agar. Thus PCDHs are candidate tumor suppressors that modulate regulatory pathways critical in development and disease, such as canonical Wnt signaling.
date: 2009-11-26
publisher: PUBLIC LIBRARY SCIENCE
official_url: http://dx.doi.org/10.1371/journal.pgen.1000745
oa_status: green
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_batch
elements_source: Web of Science
elements_id: 223721
doi: 10.1371/journal.pgen.1000745
language_elements: EN
lyricists_name: Pritchard-Jones, Kathryn
lyricists_id: KPPRI20
full_text_status: public
publication: PLOS GENET
volume: 5
number: 11
article_number: e1000745
issn: 1553-7390
citation:        Dallosso, AR;    Hancock, AL;    Szemes, M;    Moorwood, K;    Chilukamarri, L;    Tsai, HH;    Sarkar, A;                                                     ... Malik, K; + view all <#>        Dallosso, AR;  Hancock, AL;  Szemes, M;  Moorwood, K;  Chilukamarri, L;  Tsai, HH;  Sarkar, A;  Barasch, J;  Vuononvirta, R;  Jones, C;  Pritchard-Jones, K;  Royer-Pokora, B;  Lee, SB;  Owen, C;  Malik, S;  Feng, Y;  Frank, M;  Ward, A;  Brown, KW;  Malik, K;   - view fewer <#>    (2009)    Frequent Long-Range Epigenetic Silencing of Protocadherin Gene Clusters on Chromosome 5q31 in Wilms' Tumor.                   PLOS GENET , 5  (11)    , Article e1000745.  10.1371/journal.pgen.1000745 <https://doi.org/10.1371/journal.pgen.1000745>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/358409/1/358409.pdf