eprintid: 29058 rev_number: 78 eprint_status: archive userid: 608 dir: disk0/00/02/90/58 datestamp: 2010-10-14 23:37:16 lastmod: 2020-02-13 03:51:46 status_changed: 2010-10-14 23:37:16 type: article metadata_visibility: show item_issues_count: 0 creators_name: Perez-Sanchez, C creators_name: Budhram-Mahadeo, VS creators_name: Latchman, DS title: Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter ispublished: pub divisions: UCL divisions: A01 divisions: B02 divisions: C09 divisions: D14 divisions: GA2 divisions: D13 divisions: G28 keywords: Animals, Base Sequence, Binding Sites, Cell Line, Cyclin-Dependent Kinase Inhibitor p21, Cyclins, DNA-Binding Proteins, Luciferases, Molecular Sequence Data, Mutation, Neurons, Promoter Regions, Genetic, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Response Elements, Transcription Factor Brn-3, Transcription Factors, Transcriptional Activation, Tumor Suppressor Protein p53, bcl-2-Associated X Protein note: PMCID: PMC137158. This article was original published in Nucleic Acids Research by Oxford University Press, 2002 abstract: Although the promoters of both the Bax and p21 genes are activated by p53, they differ in the effect on this activation of the POU family transcription factor Brn-3a. Thus, Brn-3a inhibits activation of the Bax promoter by p53 but enhances the ability of p53 to activate the p21 promoter. We demonstrate that repression of p53-mediated activation of the Bax promoter involves a complex upstream sequence in which two Brn-3a response elements flank the p53 response element. In contrast, a minimal p21 promoter is activated by Brn-3a and such activation cannot be abolished without abolishing basal promoter activity. Moreover, synergistic activation by Brn-3a and p53 continues to be observed when the p53-binding sites in the p21 promoter are substituted by the Bax p53 site or by the region of the Bax promoter essential for Brn-3a-mediated repression, indicating that the p21 core promoter plays a central role in this response. The significance of these effects is discussed in terms of the different responses of the Bax and p21 promoters and the overlapping but distinct roles of Brn-3a and p53 in neuronal growth arrest and apoptosis. date: 2002-11-15 official_url: http://dx.doi.org/ 10.1093/nar/gkf610 vfaculties: VFPHS oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Journal Article, Research Support, Non-U.S. Gov't verified: verified_manual elements_source: PubMed elements_id: 26772 doi: 10.1093/nar/gkf610 lyricists_name: Latchman, David lyricists_name: Mahadeo, Vishwanie lyricists_id: DSLAT86 lyricists_id: VBUDH48 full_text_status: public publication: Nucleic Acids Res volume: 30 number: 22 pagerange: 4872 - 4880 event_location: England issn: 0305-1048 citation: Perez-Sanchez, C; Budhram-Mahadeo, VS; Latchman, DS; (2002) Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter. Nucleic Acids Res , 30 (22) 4872 - 4880. 10.1093/nar/gkf610 <https://doi.org/10.1093/nar%2Fgkf610>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/29058/1/Nucl._Acids_Res.-2002-Perez_Sanchez-4872-80.pdf