eprintid: 29058
rev_number: 78
eprint_status: archive
userid: 608
dir: disk0/00/02/90/58
datestamp: 2010-10-14 23:37:16
lastmod: 2020-02-13 03:51:46
status_changed: 2010-10-14 23:37:16
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Perez-Sanchez, C
creators_name: Budhram-Mahadeo, VS
creators_name: Latchman, DS
title: Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter
ispublished: pub
divisions: UCL
divisions: A01
divisions: B02
divisions: C09
divisions: D14
divisions: GA2
divisions: D13
divisions: G28
keywords: Animals, Base Sequence, Binding Sites, Cell Line, Cyclin-Dependent Kinase Inhibitor p21, Cyclins, DNA-Binding Proteins, Luciferases, Molecular Sequence Data, Mutation, Neurons, Promoter Regions, Genetic, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Response Elements, Transcription Factor Brn-3, Transcription Factors, Transcriptional Activation, Tumor Suppressor Protein p53, bcl-2-Associated X Protein
note: PMCID: PMC137158. This article was original published in Nucleic Acids Research by Oxford University Press, 2002
abstract: Although the promoters of both the Bax and p21 genes are activated by p53, they differ in the effect on this activation of the POU family transcription factor Brn-3a. Thus, Brn-3a inhibits activation of the Bax promoter by p53 but enhances the ability of p53 to activate the p21 promoter. We demonstrate that repression of p53-mediated activation of the Bax promoter involves a complex upstream sequence in which two Brn-3a response elements flank the p53 response element. In contrast, a minimal p21 promoter is activated by Brn-3a and such activation cannot be abolished without abolishing basal promoter activity. Moreover, synergistic activation by Brn-3a and p53 continues to be observed when the p53-binding sites in the p21 promoter are substituted by the Bax p53 site or by the region of the Bax promoter essential for Brn-3a-mediated repression, indicating that the p21 core promoter plays a central role in this response. The significance of these effects is discussed in terms of the different responses of the Bax and p21 promoters and the overlapping but distinct roles of Brn-3a and p53 in neuronal growth arrest and apoptosis.
date: 2002-11-15
official_url: http://dx.doi.org/ 10.1093/nar/gkf610
vfaculties: VFPHS
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article, Research Support, Non-U.S. Gov't
verified: verified_manual
elements_source: PubMed
elements_id: 26772
doi: 10.1093/nar/gkf610
lyricists_name: Latchman, David
lyricists_name: Mahadeo, Vishwanie
lyricists_id: DSLAT86
lyricists_id: VBUDH48
full_text_status: public
publication: Nucleic Acids Res
volume: 30
number: 22
pagerange: 4872 - 4880
event_location: England
issn: 0305-1048
citation:        Perez-Sanchez, C;    Budhram-Mahadeo, VS;    Latchman, DS;      (2002)    Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter.                   Nucleic Acids Res , 30  (22)   4872 - 4880.    10.1093/nar/gkf610 <https://doi.org/10.1093/nar%2Fgkf610>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/29058/1/Nucl._Acids_Res.-2002-Perez_Sanchez-4872-80.pdf