eprintid: 176618 rev_number: 184 eprint_status: archive userid: 608 dir: disk0/00/17/66/18 datestamp: 2010-11-04 18:31:02 lastmod: 2021-12-10 23:39:19 status_changed: 2013-05-29 13:53:44 type: article metadata_visibility: show item_issues_count: 0 creators_name: Wood, AJ creators_name: Roberts, RG creators_name: Monk, D creators_name: Moore, GE creators_name: Schulz, R creators_name: Oakey, RJ title: A screen for retrotransposed imprinted genes reveals an association between X chromosome homology and maternal germ-line methylation ispublished: pub divisions: UCL divisions: B02 divisions: D13 keywords: 10, 2, A, AND, Animals, ARTICLE, Aspartic Endopeptidases, Base Sequence, CpG Islands, DNA Methylation, Exons, Female, FOR, genetics, Genomic Imprinting, Human, Humans, Introns, JOURNAL, London, metabolism, Methylation, Mice, Molecular Sequence Data, Nerve Tissue Proteins, OF, Oncogene Proteins, Ovum, Phosphoric Monoester Hydrolases, Phylogeny, Proteins, Retroelements, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, THE, United Kingdom, X Chromosome note: © 2007 Wood et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. This work was supported by the Wellcome Trust and the Biotechnology and Biological Sciences Research Council (RJO), the Generation Trust (AW), and the European Molecular Biology Organization (RS). abstract: Imprinted genes undergo epigenetic modifications during gametogenesis, which lead to transcriptional silencing of either the maternally or the paternally derived allele in the subsequent generation. Previous work has suggested an association between imprinting and the products of retrotransposition, but the nature of this link is not well defined. In the mouse, three imprinted genes have been described that originated by retrotransposition and overlap CpG islands which undergo methylation during oogenesis. Nap1l5, U2af1-rs1, and Inpp5f_v2 are likely to encode proteins and share two additional genetic properties: they are located within introns of host transcripts and are derived from parental genes on the X chromosome. Using these sequence features alone, we identified Mcts2, a novel candidate imprinted retrogene on mouse Chromosome 2. Mcts2 has been validated as imprinted by demonstrating that it is paternally expressed and undergoes promoter methylation during oogenesis. The orthologous human retrogenes NAP1L5, INPP5F_V2, and MCTS2 are also shown to be paternally expressed, thus delineating novel imprinted loci on human Chromosomes 4, 10, and 20. The striking correlation between imprinting and X chromosome provenance suggests that retrotransposed elements with homology to the X chromosome can be selectively targeted for methylation during mammalian oogenesis date: 2007-02-09 official_url: http://dx.doi.org/10.1371/journal.pgen.0030020 oa_status: green language: eng primo: open primo_central: open_green article_type_text: Article verified: verified_manual elements_source: Manually entered elements_id: 90183 doi: 10.1371/journal.pgen.0030020 lyricists_name: MONK, DAVID lyricists_name: Moore, Gudrun lyricists_id: DNMON39 lyricists_id: GEMOO87 full_text_status: public publication: PLoS Genetics volume: 3 number: 2 article_number: e20 issn: 1553-7390 citation: Wood, AJ; Roberts, RG; Monk, D; Moore, GE; Schulz, R; Oakey, RJ; (2007) A screen for retrotransposed imprinted genes reveals an association between X chromosome homology and maternal germ-line methylation. PLoS Genetics , 3 (2) , Article e20. 10.1371/journal.pgen.0030020 <https://doi.org/10.1371/journal.pgen.0030020>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/176618/2/176618.pdf